Analyses of chondrogenic induction of adipose mesenchymal stem cells by combined co-stimulation mediated by adenoviral gene transfer

Garza Veloz, Idalia y Romero Díaz, Víktor J. y Martínez Fierro, Margarita de la Luz y Marino Martínez, Iván Alberto y González Rodríguez, Manuel y Martínez Rodríguez, Herminia Guadalupe y Espinoza Juárez, Marcela Alejandra y Bernal Garza, Dante A. y Ortiz López, Rocío y Rojas Martinez, Augusto (2013) Analyses of chondrogenic induction of adipose mesenchymal stem cells by combined co-stimulation mediated by adenoviral gene transfer. Arthritis Research & Therapy, 15 (4). R80. ISSN 1478-6354

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URL o página oficial: http://doi.org/10.1186/ar4260

Resumen

Introduction: Adipose-derived stem cells (ASCs) have the potential to differentiate into cartilage under stimulation with some reported growth and transcriptional factors, which may constitute an alternative for cartilage replacement approaches. In this study, we analyzed the in vitro chondrogenesis of ASCs transduced with adenoviral vectors encoding insulin-like growth factor-1 (IGF-1), transforming growth factor beta-1 (TGF-b1), fibroblast growth factor-2 (FGF-2), and sex-determining region Y-box 9 (SOX9) either alone or in combinations. Methods: Aggregate cultures of characterized ovine ASCs were transduced with 100 multiplicity of infections of Ad.IGF-1, Ad.TGF-b1, Ad.FGF-2, and Ad.SOX9 alone or in combination. These were harvested at various time points for detection of cartilage-specific genes expression by quantitative real-time PCR or after 14 and 28 days for histologic and biochemical analyses detecting proteoglycans, collagens (II, I and X), and total sulfated glycosaminoglycan and collagen content, respectively. Results: Expression analyses showed that co-expression of IGF-1 and FGF-2 resulted in higher significant expression levels of aggrecan, biglycan, cartilage matrix, proteoglycan, and collagen II (all P ≤0.001 at 28 days). Aggregates cotransduced with Ad.IGF-1/Ad.FGF-2 showed a selective expression of proteoglycans and collagen II, with limited expression of collagens I and × demonstrated by histological analyses, and had significantly greater glycosaminoglycan and collagen production than the positive control (P ≤0.001). Western blot analyses for this combination also demonstrated increased expression of collagen II, while expression of collagens I and × was undetectable and limited, respectively. Conclusion: Combined overexpression of IGF-1/FGF-2 within ASCs enhances their chondrogenic differentiation inducing the expression of chondrogenic markers, suggesting that this combination is more beneficial than the other factors tested for the development of cell-based therapies for cartilage repair.

Tipo de elemento: Article
Palabras claves no controlados: adipose-derived stem cell, chondrogenesis, adenoviral vector, growth factors, cartilage repair
Materias: Q Ciencia > QH Historia Natural, Biología
Usuario depositante: Lic. Josimar Pulido
Creadores:
CreadorEmailORCID
Garza Veloz, IdaliaNO ESPECIFICADONO ESPECIFICADO
Romero Díaz, Víktor J.NO ESPECIFICADONO ESPECIFICADO
Martínez Fierro, Margarita de la LuzNO ESPECIFICADONO ESPECIFICADO
Marino Martínez, Iván AlbertoNO ESPECIFICADONO ESPECIFICADO
González Rodríguez, ManuelNO ESPECIFICADONO ESPECIFICADO
Martínez Rodríguez, Herminia GuadalupeNO ESPECIFICADONO ESPECIFICADO
Espinoza Juárez, Marcela AlejandraNO ESPECIFICADONO ESPECIFICADO
Bernal Garza, Dante A.NO ESPECIFICADONO ESPECIFICADO
Ortiz López, RocíoNO ESPECIFICADONO ESPECIFICADO
Rojas Martinez, AugustoNO ESPECIFICADONO ESPECIFICADO
Fecha del depósito: 04 Sep 2020 14:53
Última modificación: 17 Ago 2021 20:11
URI: http://eprints.uanl.mx/id/eprint/14748

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