Recombinant protein production data after expression in the bacterium Escherichia coli

Cantú Bustos, Jesús Enrique y Cano del Villar, Kevin D. y Vargas Cortez, Teresa y Morones Ramírez, José Rubén y Balderas Rentería, Isaías y Zarate, Xristo (2016) Recombinant protein production data after expression in the bacterium Escherichia coli. Data in Brief, 7. pp. 502-508. ISSN 23523409

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Resumen

Fusion proteins have become essential for the expression and purification of recombinant proteins in Escherichia coli. The metalbinding protein CusF has shown several features that make it an attractive fusion protein and affinity tag: "Expression and purification of recombinant proteins in Escherichia coli tagged with the metalbinding protein CusF" (Cantu-Bustos et al., 2016 [1]). Here we present accompanying data from protein expression experiments; we tested different protein tags, temperatures, expression times, cellular compartments, and concentrations of inducer in order to obtain soluble protein and low formation of inclusion bodies. Additionally, we present data from the purification of the green fluorescent protein (GFP) tagged with CusF, using Ag(I) metal affinity chromatography

Tipo de elemento: Article
Palabras claves no controlados: Fusion protein, Affinity tag, Escherichia coli, CusF, GST
Divisiones: Ciencias Químicas
Usuario depositante: Lic. Jesús E. Alvarado
Creadores:
CreadorEmailORCID
Cantú Bustos, Jesús EnriqueNO ESPECIFICADONO ESPECIFICADO
Cano del Villar, Kevin D.NO ESPECIFICADONO ESPECIFICADO
Vargas Cortez, TeresaNO ESPECIFICADONO ESPECIFICADO
Morones Ramírez, José RubénNO ESPECIFICADONO ESPECIFICADO
Balderas Rentería, IsaíasNO ESPECIFICADONO ESPECIFICADO
Zarate, XristoNO ESPECIFICADONO ESPECIFICADO
Fecha del depósito: 03 Jun 2019 15:16
Última modificación: 12 Jun 2020 18:53
URI: http://eprints.uanl.mx/id/eprint/14790

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