A rapid method for isolation of total DNA from pathogenic filamentous plant fungi

Gonzlez Mendoza, D. y Argumedo Delira, Rosalba y Morales Trejo, A. y Pulido Herrera, A. y Cervantes Daz, L. y Grimaldo Juarez, O. y Alarcn, A. (2010) A rapid method for isolation of total DNA from pathogenic filamentous plant fungi. Genetics and Molecular Research, 9 (1). pp. 162-166. ISSN 16765680

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URL o página oficial: http://doi.org/10.4238/vol9-1gmr680

Resumen

DNA isolation from some fungal organisms of agronomic importance is difficult because they have cell walls or capsules that are relatively unsusceptible to lysis. We have developed a fast DNA isolation protocol for Fusarium oxysporum, which causes fusarium wilt disease in more than 100 plant species, and for Pyrenochaeta terrestris, which causes pink root in onions. This protocol was based on the sodium dodecyl sulfate/ phenol method, without β-mercaptoethanol and without maceration in liquid nitrogen; it uses phenol/chloroform extraction to remove proteins and co-precipitated polysaccharides. The A260/280 absorbance ratios of isolated DNA were around 1.9, suggesting that the DNA fraction was pure and may be used for further analysis. Additionally, the A260/230 values were higher than 1.8, suggesting negligible contamination by polysaccharides. The DNA isolated by this protocol is of sufficient quality for molecular applications; this technique could be applied to other organisms that have similar substances that hinder DNA extraction.

Tipo de elemento: Article
Palabras claves no controlados: Genomic DNA extraction; Fusarium oxysporum; Pyrenochaeta terrestris; Polymerase chain reaction; Filamentous fungiIsolation of total DNA from pathogenic filamentous plant fungi
Usuario depositante: Lic. Josimar Pulido
Creadores:
CreadorEmailORCID
Gonzlez Mendoza, D.NO ESPECIFICADONO ESPECIFICADO
Argumedo Delira, RosalbaNO ESPECIFICADONO ESPECIFICADO
Morales Trejo, A.NO ESPECIFICADONO ESPECIFICADO
Pulido Herrera, A.NO ESPECIFICADONO ESPECIFICADO
Cervantes Daz, L.NO ESPECIFICADONO ESPECIFICADO
Grimaldo Juarez, O.NO ESPECIFICADONO ESPECIFICADO
Alarcn, A.NO ESPECIFICADONO ESPECIFICADO
Fecha del depósito: 29 Abr 2019 18:41
Última modificación: 04 Jul 2019 15:15
URI: http://eprints.uanl.mx/id/eprint/15087

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