“CLUPS”: A New Culture Medium for the Axenic Growth of Entamoeba histolytica

Gonzalez Salazar, F. y Meester, Irene y Guzmán De La Garza, F. J. y De La Garza Salinas, L. H. y Sampayo Reyes, Adriana y Garza Gonzalez, J. N. y Monsivais Diaz, O. y Barba Dávila, B. A. y Hernández García, M. E. y Vargas Villarreal, Javier (2018) “CLUPS”: A New Culture Medium for the Axenic Growth of Entamoeba histolytica. Journal of Parasitology Research, 2018. pp. 1-6. ISSN 2090-0023

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URL o página oficial: http://doi.org/10.1155/2018/2796516

Resumen

Amebiasis remains a major health problem in Mexico. Terefore, the search for better culture media and low-cost diagnostic and therapeutic tools is fundamental. We present a new culture medium for Entamoeba histolytica which allows the microbe to preserve its virulence factors and ability to induce hepatic abscesses in animal models. Te novel CLUPS medium is an improved version of the PEHPS medium, previously designed in our laboratory. Te main diference is the substitution of raw beef liver in PEHPS by raw beef lung in the CLUPS medium. To compare the performance of three-culture media (traditional TYI-S-33, PEHPS, and CLUPS), E. histolytica trophozoites were cultured in quintuplicate, followed by the evaluation of phospholipase activity and the induction of liver abscesses in golden hamsters. E. histolytica trophozoites grew signifcantly better in CLUPS medium than in TYIS-33. Likewise, CLUPS-cultured trophozoites produced signifcantly more phospholipases than TYI-S-33-cultured trophozoites. Finally, trophozoites grown in any of the three tested media had similar potential to induce liver abscesses

Tipo de elemento: Article
Materias: R Medicina > RC Medicina Interna, Psiquiatría, Neurología
Usuario depositante: Lic. Josimar Pulido
Creadores:
CreadorEmailORCID
Gonzalez Salazar, F.NO ESPECIFICADONO ESPECIFICADO
Meester, IreneNO ESPECIFICADONO ESPECIFICADO
Guzmán De La Garza, F. J.NO ESPECIFICADONO ESPECIFICADO
De La Garza Salinas, L. H.NO ESPECIFICADONO ESPECIFICADO
Sampayo Reyes, AdrianaNO ESPECIFICADONO ESPECIFICADO
Garza Gonzalez, J. N.NO ESPECIFICADONO ESPECIFICADO
Monsivais Diaz, O.NO ESPECIFICADONO ESPECIFICADO
Barba Dávila, B. A.NO ESPECIFICADONO ESPECIFICADO
Hernández García, M. E.NO ESPECIFICADONO ESPECIFICADO
Vargas Villarreal, JavierNO ESPECIFICADONO ESPECIFICADO
Fecha del depósito: 21 Mayo 2020 12:31
Última modificación: 09 Jun 2020 20:42
URI: http://eprints.uanl.mx/id/eprint/17149

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