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Abdiel Martin-Park, Mayra A. Gomez-Govea, Beatriz Lopez-Monroy, Víctor Manuel Treviño-Alvarado, María del Rosario Torres-Sepúlveda, Graciela Arelí López-Uriarte, Olga Karina Villanueva-Segura, María del Consuelo Ruiz-Herrera, Margarita de la Luz Martinez-Fierro, Ivan Delgado-Enciso, Adriana E. Flores-Suárez, Gregory S. White, Laura E. Martínez de Villarreal, Gustavo Ponce-Garcia, William C. Black, Irám Pablo Rodríguez-Sanchez
Profiles of Amino Acids and Acylcarnitines Related with Insecticide Exposure in Culex quinquefasciatus (Say)
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(may)Tj
1.9276 0 Td
(result)Tj
2.4491 0 Td
(from)Tj
2.2053 0 Td
(altered)Tj
2.948 0 Td
(biochemical)Tj
5.0627 0 Td
(pathways)Tj
3.9344 0 Td
(due)Tj
1.6951 0 Td
(to)Tj
1.0262 0 Td
(alterations)Tj
-32.9837 -1.2983 Td
(or)Tj
1.0941 0 Td
(disease)Tj
3.0217 0 Td
([)Tj
0.83 0.64 0.02 0 k
(49)Tj
0 g
(].)Tj
2.075 0 Td
(Changes)Tj
3.6453 0 Td
(in)Tj
1.0318 0 Td
(acylcarnitines)Tj
5.726 0 Td
(and)Tj
1.7291 0 Td
(amino)Tj
2.7949 0 Td
(acid)Tj
1.8709 0 Td
(profiles)Tj
3.2031 0 Td
(are)Tj
1.4457 0 Td
(a)Tj
0.6576 0 Td
(powerful)Tj
3.7814 0 Td
(diagnostic)Tj
-32.0766 -1.3039 Td
(tool)Tj
1.7744 0 Td
(for)Tj
1.389 0 Td
(metabolic)Tj
4.1442 0 Td
(alterations)Tj
4.4107 0 Td
([)Tj
0.83 0.64 0.02 0 k
(50)Tj
0 g
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2.0749 0 Td
(and)Tj
1.7348 0 Td
(could)Tj
2.4492 0 Td
(be)Tj
1.1508 0 Td
(useful)Tj
2.5965 0 Td
(indicators)Tj
4.2236 0 Td
(of)Tj
1.0148 0 Td
(deviations)Tj
4.3087 0 Td
(in)Tj
1.0374 0 Td
(metabolic)Tj
-32.309 -1.2983 Td
(states.)Tj
2.6191 0 Td
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4.8926 0 Td
(these)Tj
2.2564 0 Td
(changes)Tj
3.4015 0 Td
(could)Tj
2.4548 0 Td
(be)Tj
1.1509 0 Td
(an)Tj
1.2019 0 Td
(opportunity)Tj
5.0343 0 Td
(to)Tj
1.0261 0 Td
(advance)Tj
3.4639 0 Td
(understanding)Tj
6.0718 0 Td
(the)Tj
1.4683 0 Td
(mech-)Tj
-35.0416 -1.2983 Td
(anisms)Tj
3.0103 0 Td
(of)Tj
1.0148 0 Td
(insecticide)Tj
4.4504 0 Td
(resistance,)Tj
4.3596 0 Td
(finding)Tj
3.1295 0 Td
(new)Tj
1.8765 0 Td
(control)Tj
3.1238 0 Td
(strategies.)Tj
4.1442 0 Td
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1.7858 0 Td
(objective)Tj
3.7814 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
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-33.1594 -1.3039 Td
(study)Tj
2.3867 0 Td
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1.7008 0 Td
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1.0261 0 Td
(measure)Tj
3.5773 0 Td
(the)Tj
1.474 0 Td
(amino)Tj
2.795 0 Td
(acid)Tj
1.8708 0 Td
(and)Tj
1.7291 0 Td
(acylcarnitine)Tj
5.3632 0 Td
(concentrations)Tj
6.1795 0 Td
(in)Tj
1.0374 0 Td
(larvae)Tj
2.5795 0 Td
(of)Tj
/F14 1 Tf
1.0148 0 Td
[()]TJ
/F9 1 Tf
(.)Tj
/F14 1 Tf
1.5364 0 Td
[(\n)14()12()12()]TJ
-34.2706 -1.2982 Td
[(\n)14()4()1()9(\r )-3()1()4(\r)22()7()-4( )]TJ
/F9 1 Tf
4.9776 0 Td
(after)Tj
2.0352 0 Td
(the)Tj
1.4684 0 Td
(exposure)Tj
3.8154 0 Td
(to)Tj
1.0261 0 Td
(three)Tj
2.2677 0 Td
(different)Tj
3.651 0 Td
(insecticides,)Tj
5.04 0 Td
(two)Tj
1.7121 0 Td
(organophosphates)Tj
7.5231 0 Td
(and)Tj
1.7348 0 Td
(one)Tj
-35.2514 -1.2983 Td
(pyrethroid.)Tj
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Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 511.0299 cm
BT
/F3 1 Tf
1 TL
-0.0033 Tc
0 0 Td
(Materials)Tj
4.5071 0 Td
(and)Tj
1.989 0 Td
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/F0 1 Tf
-6.4961 -1.4976 Td
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4.2283 0 Td
(sampling)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 476.0503 cm
BT
/F14 1 Tf
1 TL
0.003 Tc
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[()13()4()4()1()]TJ
2.4944 0 Td
[(\n)14()12()12()6(\n)14()4()1()9(\r )-3()1()4(\r)22()7()-4( )]TJ
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3.2428 0 Td
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1.7178 0 Td
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-34.7241 -1.2982 Td
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4.473 0 Td
(\(30x40cm\))Tj
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2.0296 0 Td
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2.0182 0 Td
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3.2031 0 Td
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1.0262 0 Td
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(food)Tj
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(source)Tj
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(for)Tj
1.4117 0 Td
(the)Tj
1.491 0 Td
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2.4435 0 Td
(stage.)Tj
2.4717 0 Td
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2.2677 0 Td
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(under)Tj
-33.2104 -1.2983 Td
(laboratory)Tj
4.422 0 Td
(conditions)Tj
4.541 0 Td
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(a)Tj
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(of)Tj
1.0261 0 Td
(27)Tj
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1.1849 0 Td
[()]TJ
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0.7993 0 Td
(2C,)Tj
2.0013 0 Td
(relative)Tj
3.1974 0 Td
(humidity)Tj
3.9629 0 Td
(of)Tj
1.0318 0 Td
(75)Tj
/F15 1 Tf
1.1848 0 Td
[()]TJ
/F9 1 Tf
0.7937 0 Td
(2%)Tj
1.4684 0 Td
(and)Tj
1.7518 0 Td
(a)Tj
0.6633 0 Td
(LD)Tj
-34.8659 -1.2983 Td
(12:12)Tj
2.381 0 Td
(h)Tj
0.754 0 Td
(photoperiod)Tj
5.2668 0 Td
([)Tj
0.83 0.64 0.02 0 k
(51)Tj
0 g
(],)Tj
2.1146 0 Td
(until)Tj
2.143 0 Td
(adult)Tj
2.2904 0 Td
(emergence.)Tj
4.8359 0 Td
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4.8018 0 Td
(of)Tj
1.0261 0 Td
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Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 461.4803 422.3622 cm
BT
/F9 1 Tf
1 TL
0.004 Tc
0 0 Td
(1)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 467.3196 424.0629 cm
BT
/F9 1 Tf
1 TL
0.003 Tc
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(generation)Tj
4.575 0 Td
(were)Tj
2.1544 0 Td
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2.0862 0 Td
(for)Tj
-35.5461 -1.3039 Td
(all)Tj
1.1679 0 Td
(the)Tj
1.491 0 Td
(assays.)Tj
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Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 383.6976 cm
BT
/F0 1 Tf
1 TL
-0.0033 Tc
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3.2362 0 Td
(bioassay)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 366.6897 cm
BT
/F14 1 Tf
1 TL
-0.007 Tc
0 0 Td
[()13()4()4()1()]TJ
2.4434 0 Td
[(\n)14()12()12()6(\n)14()4()1()9(\r )-3()1()4(\r)22()7()-4( )]TJ
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6.7294 0 Td
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2.5682 0 Td
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(exposed)Tj
3.4243 0 Td
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1.0204 0 Td
(standard)Tj
3.6964 0 Td
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3.8834 0 Td
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2.3698 0 Td
(the)Tj
1.4626 0 Td
(diagnostic)Tj
4.2917 0 Td
(dose)Tj
-33.9985 -1.2982 Td
(\(DD\))Tj
2.3584 0 Td
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1.0091 0 Td
(the)Tj
1.4627 0 Td
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3.7813 0 Td
(grade)Tj
2.4322 0 Td
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4.9946 0 Td
(chlorpyrifos)Tj
5.0286 0 Td
(99.5%)Tj
2.6135 0 Td
(\(0.001)Tj
/F15 1 Tf
2.6703 0 Td
[()]TJ
/F9 1 Tf
(g/mL\),)Tj
3.4242 0 Td
(temephos)Tj
4.0705 0 Td
(97.5%)Tj
-33.8454 -1.2983 Td
(\(0.002)Tj
/F15 1 Tf
2.6702 0 Td
[()]TJ
/F9 1 Tf
(g/mL\))Tj
3.1974 0 Td
(and)Tj
1.7235 0 Td
(permethrin)Tj
4.7565 0 Td
(99.5%)Tj
2.6192 0 Td
(\(0.01)Tj
/F15 1 Tf
2.194 0 Td
[()]TJ
/F9 1 Tf
(g/mL\))Tj
3.2031 0 Td
(\(ChemService,)Tj
6.0208 0 Td
(West)Tj
2.2563 0 Td
(Chester,)Tj
3.4866 0 Td
(PA\))Tj
1.7972 0 Td
([)Tj
0.83 0.64 0.02 0 k
(52)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
1.7234 0 Td
(53)Tj
0 g
(].)Tj
-35.6482 -1.3039 Td
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2.5625 0 Td
(replicates)Tj
3.9514 0 Td
(of)Tj
1.0092 0 Td
(each)Tj
2.0182 0 Td
(insecticide)Tj
4.4164 0 Td
(were)Tj
2.1146 0 Td
(prepared)Tj
3.7814 0 Td
(in)Tj
1.0261 0 Td
(standard)Tj
3.702 0 Td
(\(weight/volume\))Tj
6.8258 0 Td
(alcohol)Tj
3.0954 0 Td
(solu-)Tj
-34.503 -1.2983 Td
(tion.)Tj
2.0522 0 Td
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3.3392 0 Td
(groups)Tj
2.948 0 Td
(were)Tj
2.1147 0 Td
(placed)Tj
2.7722 0 Td
(in)Tj
1.0318 0 Td
(recipients)Tj
4.0876 0 Td
(containing)Tj
4.5014 0 Td
(water)Tj
2.4151 0 Td
(and)Tj
1.7234 0 Td
(1)Tj
0.6917 0 Td
(mL)Tj
1.5703 0 Td
(of)Tj
1.0092 0 Td
(alcohol.)Tj
3.3108 0 Td
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2.2734 0 Td
(24)Tj
1.1622 0 Td
(h)Tj
-37.0032 -1.2982 Td
(of)Tj
1.0091 0 Td
(insecticide)Tj
4.4163 0 Td
(exposure)Tj
3.7928 0 Td
(all)Tj
1.1395 0 Td
(larvae)Tj
2.5625 0 Td
(that)Tj
1.7688 0 Td
(were)Tj
2.109 0 Td
(found)Tj
2.6021 0 Td
(alive)Tj
2.0296 0 Td
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2.1147 0 Td
(separated)Tj
3.9798 0 Td
(from)Tj
2.194 0 Td
(dead)Tj
2.1203 0 Td
(ones.)Tj
2.2734 0 Td
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-34.1119 -1.3039 Td
(alive)Tj
2.0295 0 Td
(larvae)Tj
2.5625 0 Td
(were)Tj
2.1147 0 Td
(placed)Tj
2.7722 0 Td
(individually)Tj
4.955 0 Td
(in)Tj
1.0261 0 Td
(1.5)Tj
1.389 0 Td
(mL)Tj
1.5704 0 Td
(tubes)Tj
2.3244 0 Td
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0.9467 0 Td
(-80C)Tj
2.5625 0 Td
(for)Tj
1.3777 0 Td
(the)Tj
1.457 0 Td
(metabolomics)Tj
5.7996 0 Td
(analysis.)Tj
3.5263 0 Td
(At)Tj
-36.4136 -1.2983 Td
(the)Tj
1.4569 0 Td
(same)Tj
2.2507 0 Td
(time)Tj
2.0126 0 Td
(and)Tj
1.7235 0 Td
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1.0091 0 Td
(the)Tj
1.457 0 Td
(same)Tj
2.2507 0 Td
(way,)Tj
2.0069 0 Td
(unexposed)Tj
4.4901 0 Td
(larvae)Tj
2.5681 0 Td
(of)Tj
/F14 1 Tf
1.0092 0 Td
[()]TJ
/F9 1 Tf
(.)Tj
/F14 1 Tf
1.5193 0 Td
[(\n)14()12()12()6(\n)14()4()1()9(\r )-3()1()4(\r)22()7()-4( )]TJ
/F9 1 Tf
6.7294 0 Td
(were)Tj
2.1147 0 Td
(stored.)Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 248.3716 cm
BT
/F0 1 Tf
1 TL
-0.0033 Tc
0 0 Td
(Preparation)Tj
5.3244 0 Td
(of)Tj
1.0441 0 Td
(samples)Tj
3.9024 0 Td
(and)Tj
1.8662 0 Td
(extraction)Tj
4.5071 0 Td
(of)Tj
1.0441 0 Td
(metabolites)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 231.3637 cm
BT
/F9 1 Tf
1 TL
-0.004 Tc
0 0 Td
(Three)Tj
2.5738 0 Td
(replicates)Tj
3.9855 0 Td
(of)Tj
1.0148 0 Td
(10)Tj
1.1735 0 Td
(live)Tj
1.6101 0 Td
(larvae)Tj
2.5852 0 Td
(were)Tj
2.1203 0 Td
(homogenized)Tj
5.6522 0 Td
(in)Tj
1.0375 0 Td
(500)Tj
/F15 1 Tf
1.6441 0 Td
[()]TJ
/F9 1 Tf
(L)Tj
1.2642 0 Td
(of)Tj
1.0148 0 Td
(ddH)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 475.0299 229.6629 cm
BT
/F9 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(2)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 478.6015 231.3637 cm
BT
/F9 1 Tf
1 TL
-0.004 Tc
0 0 Td
(O)Tj
0.9637 0 Td
(\(unexposed)Tj
4.8642 0 Td
(and)Tj
-33.6866 -1.3039 Td
(exposed\))Tj
3.7814 0 Td
(and)Tj
1.7348 0 Td
(placed)Tj
2.7893 0 Td
(in)Tj
1.0375 0 Td
(1.5)Tj
1.3946 0 Td
(mL)Tj
1.5817 0 Td
(Eppendorf)Tj
4.4901 0 Td
(tubes)Tj
2.3357 0 Td
(and)Tj
1.7291 0 Td
(centrifuged)Tj
4.7849 0 Td
(for)Tj
1.3833 0 Td
(10)Tj
1.1734 0 Td
(seconds.)Tj
3.6 0 Td
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1.7801 0 Td
(lysate)Tj
2.4378 0 Td
(was)Tj
-36.0337 -1.2982 Td
(recovered)Tj
4.1443 0 Td
(with)Tj
1.9955 0 Td
(a)Tj
0.6577 0 Td
(sterile)Tj
2.6078 0 Td
(syringe)Tj
3.1124 0 Td
(in)Tj
1.0375 0 Td
(which)Tj
2.6476 0 Td
(was)Tj
1.7007 0 Td
(attached)Tj
3.5887 0 Td
(one)Tj
1.6951 0 Td
(0.22)Tj
1.8765 0 Td
(uM)Tj
1.6384 0 Td
(acrodisc.)Tj
3.7586 0 Td
(The)Tj
1.7859 0 Td
(filtrate)Tj
2.8459 0 Td
(was)Tj
-35.0926 -1.2983 Td
(placed)Tj
2.7893 0 Td
(in)Tj
1.0375 0 Td
(another)Tj
3.3278 0 Td
(tube.)Tj
2.2054 0 Td
(Finally,)Tj
3.1691 0 Td
(30)Tj
/F15 1 Tf
1.1735 0 Td
[()]TJ
/F9 1 Tf
(L)Tj
1.2643 0 Td
(from)Tj
2.2053 0 Td
(the)Tj
1.4683 0 Td
(filtered)Tj
3.0671 0 Td
(lysate)Tj
2.4378 0 Td
(was)Tj
1.6951 0 Td
(added)Tj
2.6644 0 Td
(using)Tj
2.3868 0 Td
(filter)Tj
2.1146 0 Td
(paper)Tj
-33.0063 -1.2983 Td
(\(S&S903\).)Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 151.9937 cm
BT
/F0 1 Tf
1 TL
-0.0033 Tc
0 0 Td
(Metabolomics)Tj
6.3213 0 Td
(analysis)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 134.9858 cm
BT
/F9 1 Tf
1 TL
-0.004 Tc
0 0 Td
(Each)Tj
2.1713 0 Td
(sample)Tj
3.0217 0 Td
(was)Tj
1.7008 0 Td
(analyzed)Tj
3.702 0 Td
(for)Tj
1.3833 0 Td
(12)Tj
1.1735 0 Td
(amino)Tj
2.795 0 Td
(acids)Tj
2.2337 0 Td
(and)Tj
1.7291 0 Td
(31)Tj
1.1735 0 Td
(acylcarnitines)Tj
5.7203 0 Td
([a)Tj
0.9978 0 Td
(3.2)Tj
1.3946 0 Td
(mm)Tj
1.8595 0 Td
(circle)Tj
2.3755 0 Td
(was)Tj
-33.4316 -1.2982 Td
(obtained)Tj
3.7303 0 Td
(using)Tj
2.3924 0 Td
(a)Tj
0.652 0 Td
(Wallac)Tj
2.9707 0 Td
(DBS)Tj
2.0126 0 Td
(Puncher)Tj
3.6 0 Td
(\(PerkinElmer,)Tj
5.879 0 Td
(Waltham,)Tj
4.1726 0 Td
(MA,)Tj
2.0239 0 Td
(USA\)])Tj
2.7949 0 Td
(from)Tj
2.2054 0 Td
(the)Tj
1.4683 0 Td
(dry)Tj
1.5761 0 Td
(filter)Tj
-35.4782 -1.2983 Td
(paper.)Tj
2.7099 0 Td
(A)Tj
0.9014 0 Td
(NeoBase)Tj
3.685 0 Td
(non-derivatized)Tj
6.582 0 Td
(LC-MS/MS)Tj
4.8415 0 Td
(kit)Tj
1.2813 0 Td
(\(Perkin)Tj
3.2258 0 Td
(Elmer\))Tj
2.9877 0 Td
(was)Tj
1.7008 0 Td
(used)Tj
2.0579 0 Td
(to)Tj
1.0261 0 Td
(obtain)Tj
2.7836 0 Td
(the)Tj
-33.783 -1.3039 Td
(metabolites)Tj
4.8018 0 Td
(of)Tj
1.0148 0 Td
(interest,)Tj
3.4412 0 Td
(following)Tj
3.9799 0 Td
(the)Tj
1.4683 0 Td
(manufacturer's)Tj
6.2645 0 Td
(instructions.)Tj
5.2214 0 Td
(A)Tj
0.9071 0 Td
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