Engineering brown fat into skeletal muscle using ultrasound-targeted microbubble destruction gene delivery in obese Zucker rats: Proof of concept design

Bastarrachea, Raúl A. y Chen, Jiaxi y Kent, Jack W. y Nava González, Edna Judith y Rodríguez Ayala, Ernesto y Daadi, Marcel M. y Jorge, Barbara y Laviada Molina, Hugo A. y Comuzzie, Anthony G. y Chen, Shuyuan y Grayburn, Paul A. (2017) Engineering brown fat into skeletal muscle using ultrasound-targeted microbubble destruction gene delivery in obese Zucker rats: Proof of concept design. IUBMB Life, 69 (9). pp. 745-755. ISSN 15216543

[img]
Vista previa
Texto
4.pdf - Versión Publicada
Available under License Creative Commons Attribution Non-commercial No Derivatives.

Download (1MB) | Vista previa
URL o página oficial: http://doi.org/10.1002/iub.1658

Resumen

plasmid cDNA contructs encoding a gene cocktail with BMP7/ PRDM16/PPARGC1A incorporated within microbubbles and intravenously delivered into their left thigh. Controls received UTMD with plasmids driving a DsRed reporter gene. An ultrasound transducer was directed to the thigh to disrupt the microbubbles within the microcirculation. Blood samples were drawn at baseline, and after treatment to measure glucose, insulin, and free fatty acids levels

Tipo de elemento: Article
Palabras claves no controlados: Gene therapy, UTMD, Microbubbles, Brown adipose tissue
Materias: R Medicina > RA Aspectos Públicos de la Medicina
Divisiones: Salud Pública y Nutrición
Usuario depositante: Editor Repositorio
Creadores:
CreadorEmailORCID
Bastarrachea, Raúl A.NO ESPECIFICADONO ESPECIFICADO
Chen, JiaxiNO ESPECIFICADONO ESPECIFICADO
Kent, Jack W.NO ESPECIFICADONO ESPECIFICADO
Nava González, Edna Judithedna.navag@uanl.mxNO ESPECIFICADO
Rodríguez Ayala, ErnestoNO ESPECIFICADONO ESPECIFICADO
Daadi, Marcel M.NO ESPECIFICADONO ESPECIFICADO
Jorge, BarbaraNO ESPECIFICADONO ESPECIFICADO
Laviada Molina, Hugo A.NO ESPECIFICADONO ESPECIFICADO
Comuzzie, Anthony G.NO ESPECIFICADONO ESPECIFICADO
Chen, ShuyuanNO ESPECIFICADONO ESPECIFICADO
Grayburn, Paul A.NO ESPECIFICADONO ESPECIFICADO
Fecha del depósito: 05 Mar 2020 20:31
Última modificación: 05 Mar 2020 20:31
URI: http://eprints.uanl.mx/id/eprint/18859

Actions (login required)

Ver elemento Ver elemento

Downloads

Downloads per month over past year