Successful pharmaceutical-grade streptozotocin (STZ)-induced hyperglycemia in a conscious tethered baboon (Papio hamadryas) model

Frost, Patrice A. y Chen, Shuyuan y Mezzles, Marguerite J. y Voruganti, Venkata Saroja y Nava González, Edna Judith y Arriaga Cazares, Hector E. y Freed, Katy A. y Comuzzie, Anthony G. y DeFronzo, Ralph A. y Kent, Jack W. y Grayburn, Paul A. y Bastarrachea, Raúl A. (2015) Successful pharmaceutical-grade streptozotocin (STZ)-induced hyperglycemia in a conscious tethered baboon (Papio hamadryas) model. Journal of Medical Primatology, 44 (4). pp. 202-217. ISSN 00472565

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URL o página oficial: http://doi.org/10.1111/jmp.12182

Resumen

Background Non-human primate (NHP) diabetic models using chemical ablation of b-cells with STZ have been achieved by several research groups. Chemotherapeutic STZ could lead to serious adverse events including nephrotoxicity, hepatotoxicity, and mortality. Methods We implemented a comprehensive therapeutic strategy that included the tether system, permanent indwelling catheter implants, an aggressive hydration protocol, management for pain with IV nubain and anxiety with IV midazolam, moment-by-moment monitoring of glucose levels post-STZ administration, and continuous intravenous insulin therapy. Results A triphasic response in blood glucose after STZ administration was fully characterized. A dangerous hypoglycemic phase was also detected in all baboons. Other significant findings were hyperglycemia associated with low levels of plasma leptin, insulin and C-peptide concentrations, hyperglucagonemia, and elevated non-esterified fatty acids (NEFA) concentrations. Conclusions We successfully induced frank diabetes by IV administering a single dose of pharmaceutical-grade STZ safely and without adverse events in conscious tethered baboons.

Tipo de elemento: Article
Palabras claves no controlados: Fulminant type 1 diabetes, Hydration protocol, Hyperglucagonemia, Hypoglycemia, Hypoleptinemia, Triphasic, Blood glucose response
Materias: R Medicina > RA Aspectos Públicos de la Medicina
Divisiones: Salud Pública y Nutrición
Usuario depositante: Editor Repositorio
Creadores:
CreadorEmailORCID
Frost, Patrice A.NO ESPECIFICADONO ESPECIFICADO
Chen, ShuyuanNO ESPECIFICADONO ESPECIFICADO
Mezzles, Marguerite J.NO ESPECIFICADONO ESPECIFICADO
Voruganti, Venkata SarojaNO ESPECIFICADONO ESPECIFICADO
Nava González, Edna Judithedna.navag@uanl.mxNO ESPECIFICADO
Arriaga Cazares, Hector E.NO ESPECIFICADONO ESPECIFICADO
Freed, Katy A.NO ESPECIFICADONO ESPECIFICADO
Comuzzie, Anthony G.NO ESPECIFICADONO ESPECIFICADO
DeFronzo, Ralph A.NO ESPECIFICADONO ESPECIFICADO
Kent, Jack W.NO ESPECIFICADONO ESPECIFICADO
Grayburn, Paul A.NO ESPECIFICADONO ESPECIFICADO
Bastarrachea, Raúl A.NO ESPECIFICADONO ESPECIFICADO
Fecha del depósito: 06 Mar 2020 14:57
Última modificación: 01 Mayo 2020 16:04
URI: http://eprints.uanl.mx/id/eprint/18861

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