Antibacterial and cytotoxic activities of new sphingolipids and other constituents isolated from Cissus incisa leaves

Nocedo Mena, Deyani y Rivas Galindo, Verónica M. y Navarro, Patricia y Garza González, Elvira y González Maya, Leticia y Ríos, María Yolanda y García, Abraham y Ávalos Alanís, Francisco G. y Rodríguez Rodríguez, José Antonio y Camacho Corona, María del Rayo (2020) Antibacterial and cytotoxic activities of new sphingolipids and other constituents isolated from Cissus incisa leaves. Heliyon, 6 (8). e04671. ISSN 2405-8440

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Resumen

Cissus incisa is used in traditional Mexican medicine to treat certain ailments, infectious or cancerous diseases. Excepting for our previous research, this species had no scientific reports validating its traditional use. In this study, we evaluated the antibacterial and cytotoxic properties of the sphingolipids and others phytocompounds isolated from C. incisa leaves to increase the scientific knowledge of the Mexican flora. The antibacterial activity was evaluated against Gram-positive and Gram-negative bacteria by the Microdilution method. Meanwhile, the cytotoxic potential was determined on six human cancer cells: PC3, Hep3B, HepG2, MCF7, A549, and HeLa; using an aqueous solution cell proliferation assay kit. A cell line of immortalized human hepatocytes (IHH) was included as a control of non-cancerous cells. Selectivity index (SI) was determined only against the hepatocellular carcinoma cell lines. The phytochemical investigation of C. incisa leaves resulted in the isolation and characterization of five compounds: 2-(20-hydroxydecanoyl amino)-1,3,4-hexadecanotriol-8-ene (1), 2,3-dihydroxypropyl tetracosanoate (2), β-sitosterol-D-glucopyranoside (3), α-amyrin-3-O-β-D-glucopyranoside (4), and a mixture of cerebrosides (5). Until now, this is the first report of the sphingolipids (1), (5-IV) and (5-V). Only the compound (4) and cerebrosides (5) exhibited antibacterial activity reaching a MIC value of 100 μg/mL against Pseudomonas aeruginosa resistant to carbapenems. While, the acetylated derivate of (3), compound (3Ac) showed the best cytotoxic result against PC3 (IC50 ¼ 43 � 4 μg/mL) and Hep3B (IC50 ¼ 49.0 � 4 μg/mL) cancer cell lines. Likewise, (3Ac) achieved better SI values on HepG2 and Hep3B cell lines. This research reveals the importance of study medicinal plants, to identify bioactive molecules as sources of potential drugs. The presence of these compounds allows us to justify the use of this plant in traditional Mexican medicine.

Tipo de elemento: Article
Palabras claves no controlados: Natural product chemistry, Cissus incisa, Traditional medicine, Sphingolipids, Antibacterial activity, Cytotoxic activity
Materias: Q Ciencia > QD Química
Divisiones: Ciencias Químicas
Usuario depositante: Editor Repositorio
Creadores:
CreadorEmailORCID
Nocedo Mena, DeyaniNO ESPECIFICADONO ESPECIFICADO
Rivas Galindo, Verónica M.NO ESPECIFICADONO ESPECIFICADO
Navarro, PatriciaNO ESPECIFICADONO ESPECIFICADO
Garza González, ElviraNO ESPECIFICADONO ESPECIFICADO
González Maya, LeticiaNO ESPECIFICADONO ESPECIFICADO
Ríos, María YolandaNO ESPECIFICADONO ESPECIFICADO
García, AbrahamNO ESPECIFICADONO ESPECIFICADO
Ávalos Alanís, Francisco G.NO ESPECIFICADONO ESPECIFICADO
Rodríguez Rodríguez, José AntonioNO ESPECIFICADONO ESPECIFICADO
Camacho Corona, María del RayoNO ESPECIFICADONO ESPECIFICADO
Fecha del depósito: 16 Oct 2020 12:53
Última modificación: 16 Oct 2020 12:53
URI: http://eprints.uanl.mx/id/eprint/20113

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