Modification of Branched Polyethyleneimine Using Mesquite Gum for Its Improved Hemocompatibility

Pinilla Torres, Ana M. y Carrión García, Paola Y. y Sánchez Domínguez, Celia N. y Gallardo Blanco, Hugo L y Sánchez Domínguez, Margarita (2021) Modification of Branched Polyethyleneimine Using Mesquite Gum for Its Improved Hemocompatibility. Polymers, 13 (16). pp. 1-16. ISSN 2073-4360

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URL o página oficial: http://doi.org/10.3390/polym13162766

Resumen

In the present study, the modification of branched polyethyleneimine (b-PEI) was carried out using mesquite gum (MG) to improve its hemocompatibility to be used in biomedical applications. In the copolymer synthesis process (carboxymethylated mesquite gum grafted polyethyleneimine copolymer (CBX-MG-PEI), an MG carboxymethylation reaction was initially carried out (carboxymethylated mesquite gum (CBX-MG). Subsequently, the functionalization between CBX-MG and b-PEI was carried out using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS) as crosslinking agents. The synthesis products were characterized using Fourier transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), and thermogravimetric analysis (TGA). Thermogravimetric analysis showed that CBX-MG and CBX-MG-PEI presented a lower decomposition temperature than MG. The CBX-MG-PEI has a high buffer capacity in the pH range of 4 to 7, similar to the b-PEI. In addition, the CBX-MG-PEI showed an improvement in hemocompatibility in comparison with the b-PEI. The results showed a non-hemolytic property at doses lower than 0.1 µg/mL (CBX-MG-PEI). These results allow us to propose that this copolymer be used in transfection, polymeric nanoparticles, and biomaterials due to its physicochemical and hemocompatibility properties.

Tipo de elemento:

Article

Palabras claves no controlados:

Biocompatibilidad; Polietilenimina ramificada; Capacidad amortiguadora; Funcionalización; Hemocompatibilidad; Goma de mezquite

Materias:

R Medicina > RS Farmacia y Materia Médica

Divisiones:

Medicina > Maestría en Ciencias con Orientación en Biología Molecular e Ingeniería Genética

Usuario depositante:

Dr.C. Hugo Gallardo Blanco

Creadores:

Creador	Email	ORCID
Pinilla Torres, Ana M.	anniepinillat@hotmail.com	orcid.org/0000-0002-7938-2923
Carrión García, Paola Y.	carriongarcia.paola@gmail.com	orcid.org/0009-0009-9055-152X
Sánchez Domínguez, Celia N.	celia.sanchezdm@uanl.edu.mx	orcid.org/0000-0002-3444-9565
Gallardo Blanco, Hugo L	hugo.gallardobl@uanl.edu.mx	orcid.org/0000-0002-7816-4967
Sánchez Domínguez, Margarita	margarita.sanchez@cimav.edu.mx	orcid.org/0000-0003-4755-4441