In Vitro Anthelmintic Effect of Mexican Plant Extracts and Partitions Against Trichinella spiralis and Strongyloides venezuelensis

Rodríguez Garza, Nancy Edith y Gomez Flores, Ricardo y Quintanilla Licea, Ramiro y Elizondo Luévano, Joel Horacio y Romo Sáenz, César Iván y Marín, Miguel y Sánchez Montejo, Javier y Muro, Antonio y Peláez, Rafael y López Abán, Julio (2024) In Vitro Anthelmintic Effect of Mexican Plant Extracts and Partitions Against Trichinella spiralis and Strongyloides venezuelensis. Plants, 13. pp. 1-14. ISSN 2223-7747

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URL o página oficial: https://www.mdpi.com/2223-7747/13/24/3484

Resumen

Parasitic diseases represent a significant global public health concern. Two clinically important parasites of high prevalence rates are Trichinella spiralis and Strongyloides stercoralis. However, the limitations of currently used nematocidal drugs highlight the urgent need for novel treatment approaches. The present study investigated the in vitro nematocidal activity of methanol extracts from Amphipterygium adstringens, Artemisia ludoviciana, Cymbopogon citratus, Heterotheca inuloides, Jatropha dioica, Justicia spicigera, Larrea tridentata, Mimosa tenuiflora, Psacalium decompositum, Ruta chalepensis, Semialarium mexicanum, and Smilax aspera against T. spiralis L1 and S. venezuelensis L3 (model for S. stercoralis). Most of the plants showed antiparasitic activity, but R. chalepensis crude methanol extract showed the most potent nematocidal activity against both parasites, with a mean lethal concentration (LC50) of 28.2 μg/mL and a selectivity index (SI) of 22.4 for T. spiralis and an LC50 of 244.8 μg/mL and SI of 2.58 for S. venezuelensis. This extract was further separated into n-hexane, chloroform, and methanol partitions by continuous Soxhlet extractions. The n-hexane partition demonstrated the strongest activity against both parasites, with an LC50 of 147.6 μg/mL and an SI of 7.77 against T. spiralis and an LC50 of 39.2 μg/mL and an SI of 3.77 against S. venezuelensis. LC-MS/MS analysis identified coumarins as the main chemical class (53%), and chalepin represented this partition’s most abundant compound (29.9%). Overall, this study confirmed the antiparasitic potential of medicinal plants commonly used in Mexico. In addition, it highlights the possibility of obtaining bioactive compounds from plants like R. chalepensis, or the other plants evaluated in this study, as novel treatments against parasitic diseases.

Tipo de elemento:

Article

Palabras claves no controlados:

Actividad nematocida; Actividad parasitaria; Plantas medicinales; Extracto metanólico; Trichinella spiralis; Strongyloides venezuelensis

Materias:

CONACYT > Biología y Química

Divisiones:

Ciencias Biológicas > Doctorado en Ciencias con orientación en Inmunobiología

Usuario depositante:

MC Nancy Edith Rodríguez Garza

Creadores:

Creador	Email	ORCID
Rodríguez Garza, Nancy Edith	nancy.rodriguezgrz@uanl.edu.mx	orcid.org/0000-0003-3201-5437
Gomez Flores, Ricardo	rgomez60@hotmail.com	orcid.org/0000-0002-9518-4433
Quintanilla Licea, Ramiro	ramiro.quintanillalc@uanl.edu.mx	orcid.org/0000-0002-4379-6913
Elizondo Luévano, Joel Horacio	NO ESPECIFICADO	NO ESPECIFICADO
Romo Sáenz, César Iván	cesar.romosnz@uanl.edu.mx	NO ESPECIFICADO
Marín, Miguel	NO ESPECIFICADO	NO ESPECIFICADO
Sánchez Montejo, Javier	NO ESPECIFICADO	NO ESPECIFICADO
Muro, Antonio	NO ESPECIFICADO	NO ESPECIFICADO
Peláez, Rafael	NO ESPECIFICADO	NO ESPECIFICADO
López Abán, Julio	NO ESPECIFICADO	NO ESPECIFICADO