Production of biologically active human lymphotactin (XCL1) by Lactococcus lactis

Zavala Flores, Laura Mireya y Villatoro Hernández, Julio y Gámez Escobedo, Idalia Analí y Franco Molina, Moisés Armides y Rangel Colmenero, Blanca Rocío y Villanueva Olivo, Arnulfo y Gutiérrez Puente, Yolanda y Montes de Oca Luna, Roberto y Valdés Flores, Jesús y Saucedo Cárdenas, Odila (2009) Production of biologically active human lymphotactin (XCL1) by Lactococcus lactis. Biotechnol lett, 31. pp. 215-220.

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Resumen

Lymphotactin-XCL1 is a chemokine produced mainly by activated CD8? T-cells and directs migration of CD4? and CD8? lymphocytes and natural killer (NK) cells. We expressed human lymphotactin (LTN) by the lactic-acid bacterium Lactococcus lactis. Biological activity of LTN was confirmed by chemo-attraction of human T-cells by chemotaxis demonstrating, for the first time, how this chemokine secreted by a food-grade prokaryote retains biological activity and chemoattracts T lymphocytes. This strain thus represents a feasible well-tolerated vector to deliver active LTN at a mucosal level.

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