Expression of the HPV18/E6 oncoprotein induces DNA damage

Cortés Gutiérrez, Elva Irene y García Vielma, Catalina y Aguilar Lemarroy, Adriana y Vallejo Ruíz, Veronica y Piña Sánchez, Patricia y Zapata Benavides, Pablo y Gosálvez, Jaime (2017) Expression of the HPV18/E6 oncoprotein induces DNA damage. European Journal of Histochemistry, 61 (2). pp. 96-101. ISSN 1121-760X

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Resumen

This study investigated possible variations in DNA damage in HeLa cells with silenced expression of the HPV/E6 oncogene compared with HeLa cells with normal expression of the E6 oncogene using the DNA breakage detection-fluorescence in situ hybridization (DBD-FISH) technique and a whole human genome DNA probe. The variable levels of DNA breaks present were measured quantitatively using image analysis after whole-genome DNA hybridization. HeLa cells with silenced expression of the HPV18/E6 oncogene showed a significant decrease in DNA damage compared with parental cells with normal expression of the E6 oncogene. These results were confirmed by alkaline comet assay. In conclusion, we demonstrated a decrease in DNA damage in HeLa clones associated with low expression of the HPV/E6 oncogene. The significance of this decrease regarding the HPV life cycle and carcinogenesis requires further exploration.

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