Expression of the HPV18/E6 oncoprotein induces DNA damage

Cortés Gutiérrez, Elva I. y García Vielma, Catalina y Aguilar Lemarroy, Adriana y Vallejo Ruíz, Veronica y Piña Sánchez, Patricia y Zapata Benavides, Pablo y Gosalvez, Jaime (2017) Expression of the HPV18/E6 oncoprotein induces DNA damage. European Journal of Histochemistry, 61 (2). pp. 96-101. ISSN 1121-760X

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URL o página oficial: http://doi.org/10.4081/ejh.2017.2773

Resumen

This study investigated possible variations in DNA damage in HeLa cells with silenced expression of the HPV/E6 oncogene compared with HeLa cells with normal expression of the E6 oncogene using the DNA breakage detection-fluorescence in situ hybridization (DBD-FISH) technique and a whole human genome DNA probe. The variable levels of DNA breaks present were measured quantitatively using image analysis after whole-genome DNA hybridization. HeLa cells with silenced expression of the HPV18/E6 oncogene showed a significant decrease in DNA damage compared with parental cells with normal expression of the E6 oncogene. These results were confirmed by alkaline comet assay. In conclusion, we demonstrated a decrease in DNA damage in HeLa clones associated with low expression of the HPV/E6 oncogene. The significance of this decrease regarding the HPV life cycle and carcinogenesis requires further exploration.

Tipo de elemento: Article
Palabras claves no controlados: DNA damage, DNA breakage detection-fluorescence in situ hybridization, DBD-FISH, Human papillomavirus, HPV; E6 oncogene, Alkaline-labile sites
Materias: Q Ciencia > QR Microbiología
Divisiones: Ciencias Biológicas
Usuario depositante: Editor Repositorio
Creadores:
CreadorEmailORCID
Cortés Gutiérrez, Elva I.NO ESPECIFICADONO ESPECIFICADO
García Vielma, CatalinaNO ESPECIFICADONO ESPECIFICADO
Aguilar Lemarroy, AdrianaNO ESPECIFICADONO ESPECIFICADO
Vallejo Ruíz, VeronicaNO ESPECIFICADONO ESPECIFICADO
Piña Sánchez, PatriciaNO ESPECIFICADONO ESPECIFICADO
Zapata Benavides, PabloNO ESPECIFICADONO ESPECIFICADO
Gosalvez, JaimeNO ESPECIFICADONO ESPECIFICADO
Fecha del depósito: 19 Mayo 2020 17:11
Última modificación: 19 Mayo 2020 17:11
URI: http://eprints.uanl.mx/id/eprint/14581

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