Identification and Characterization of microRNAS from Entamoeba histolytica HM1-IMSS

Rameshwar, Pranela y Mar Aguilar, Fermín y Trevino, Victor y Salinas Hernández, Jannet E. y Taméz Guerrero, Marcela M. y Barrón González, María P. y Morales Rubio, Eufemia y Treviño Neávez, Jaime y Verduzco Martínez, Jorge A. y Morales Vallarta, Mario R. y Reséndez Pérez, Diana (2013) Identification and Characterization of microRNAS from Entamoeba histolytica HM1-IMSS. PLoS ONE, 8 (7). e68202. ISSN 1932-6203

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Resumen

Background: Entamoeba histolytica is the causative agent of amebiasis, a disease that is a major source of morbidity and mortality in the developing world. MicroRNAs (miRNAs) are a large group of non-coding RNAs that play important roles in regulating gene expression and protein translation in animals. Genome-wide identification of miRNAs is a critical step to facilitating our understanding of genome organization, genome biology, evolution, and post-transcriptional regulation. Methodology/Principal Findings: We sequenced a small RNA library prepared from a culture of trophozoites of Entamoeba histolytica Strain HM1-IMSS using a deep DNA sequencing approach. Deep sequencing yielded 16 million high-quality short sequence reads containing a total of 5 million non-redundant sequence reads. Based on a bioinformatics pipeline, we found that only 0.5% of these non-redundant small RNA reads were a perfect match with the drafted E. histolytica genome. We did not find miRNA homologs in plant or animal miRNAs. We discovered 199 new potential Entamoeba histolytica miRNAs. The expression and sequence of these Ehi-miRNAs were further validated through microarray by mParaflo Microfluidic Biochip Technology. Ten potential miRNAs were additionally confirmed by real time RT-PCR analysis. Prediction of target genes matched 32 known genes and 34 hypothetical genes. Conclusions/Significance: These results show that there is a number of regulatory miRNAs in Entamoeba histolytica. The collection of miRNAs in this parasite could be used as a new platform to study genomic structure, gene regulation and networks, development, and host-parasite interactions.

Tipo de elemento: Article
Divisiones: Ciencias Biológicas
Usuario depositante: Lic. Josimar Pulido
Creadores:
CreadorEmailORCID
Rameshwar, PranelaNO ESPECIFICADONO ESPECIFICADO
Mar Aguilar, FermínNO ESPECIFICADONO ESPECIFICADO
Trevino, VictorNO ESPECIFICADONO ESPECIFICADO
Salinas Hernández, Jannet E.NO ESPECIFICADONO ESPECIFICADO
Taméz Guerrero, Marcela M.NO ESPECIFICADONO ESPECIFICADO
Barrón González, María P.NO ESPECIFICADONO ESPECIFICADO
Morales Rubio, EufemiaNO ESPECIFICADONO ESPECIFICADO
Treviño Neávez, JaimeNO ESPECIFICADONO ESPECIFICADO
Verduzco Martínez, Jorge A.NO ESPECIFICADONO ESPECIFICADO
Morales Vallarta, Mario R.NO ESPECIFICADONO ESPECIFICADO
Reséndez Pérez, DianaNO ESPECIFICADONO ESPECIFICADO
Fecha del depósito: 26 Ago 2020 02:48
Última modificación: 26 Ago 2020 02:48
URI: http://eprints.uanl.mx/id/eprint/14679

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