A Novel Gold Calreticulin Nanocomposite Based on Chitosan for Wound Healing in a Diabetic Mice Model

Hernández Martínez, Sara Paola y Rivera González, Teodoro Iván y Franco Molina, Moisés Armides y Bollain y Goytia de la Rosa, Juan José y Martínez Sanmiguel, Juan José y Zárate Triviño, Diana Ginette y Rodríguez Padilla, Cristina (2019) A Novel Gold Calreticulin Nanocomposite Based on Chitosan for Wound Healing in a Diabetic Mice Model. Nanomaterials, 9 (1). pp. 1-16. ISSN 2079-4991

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Resumen

Thedevelopmentofnewnanomaterialstopromotewoundhealingisrising,becauseoftheirtopicaladministrationandeasyfunctionalizationwithmoleculesthatcanimproveandacceleratethe processofhealing.Ananocompositeofgoldnanoparticles(AuNPs)functionalizedwithcalreticulinwassynthetizedandevaluated.Theabilityofthenanocompositetopromoteproliferationandmigrationwasdeterminedinvitro,andinvivowoundhealingwasevaluatedusingamicemodelofdiabetesestablishedwithstreptozotocin(STZ).Invitro,thenanocompositenotaffectthecellviabilityandtheexpressionofproliferatingcellnuclearantigen(PCNA).Moreover,thenanocompositepromotestheclonogenicityof keratinocytes,endothelialcells,andfibroblasts,andacceleratesfibroblastmigration.Invivo,micetreatedwiththenanocompositepresentedsignificantlyfasterwoundhealing. Thehistologicalevaluationshowed re-epithelization and the formation of granular tissue, as well as an increase of collagen deposition. Therefore,theseresultsconfirmtheutilityofAuNPs–calreticulinnanocompositesaspotentialtreatment forwoundhealingofdiabeticulcers

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