Quantification of nitric oxide by high‑performance liquid chromatography‑fluorometric method in subgenomic hepatitis C virus‑replicon expressing Huh7 cells upon treatment with acetylsalicylic acid

Ríos Ibarra, Clara Patricia y Torres de la Cruz, Víctor Manuel y Ochoa Ruiz, Andrea Gabriela y Rivas Estilla, Ana María (2018) Quantification of nitric oxide by high‑performance liquid chromatography‑fluorometric method in subgenomic hepatitis C virus‑replicon expressing Huh7 cells upon treatment with acetylsalicylic acid. Experimental and Therapeutic Medicine. pp. 2622-2626. ISSN 1792-0981

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URL o página oficial: http://doi.org/10.3892/etm.2018.6515

Resumen

As nitric oxide (NO) expression levels are lower in hepatocytes compared with other cell types, it is difficult to quantify this compound via Griess assay. The aim of the present study was to quantify NO concentration in the cell culture medium from a subgenomic hepatitis C virus (HCV)‑replicon expressing Huh‑7 cell system using a high‑performance liquid chromatography (HPLC)‑fluorescence detector in the presence or absence of acetylsalicylic acid (ASA) treatment. HCV‑replicon cells were incubated with ASA (4 mM) for 24, 48 and 72 h. Thereafter, the medium was collected to measure nitrites (NO2-) as an indirect indicator of NO levels using diaminonaphtalene as a derivate agent. NO levels were significantly higher (1.7‑fold) in Huh‑7 replicon cells treated with ASA (72 h post‑treatment) than untreated cells (P<0.05); NO inhibitor reduced ~30% the level of NO in Huh‑7 replicon cells treated with ASA (48 h post‑treatment; P<0.05). The findings suggested that the HPLC‑fluorescence method provided an accurate and efficient measurement of NO production in Huh‑7‑HCV‑replicon cells culture medium.

Tipo de elemento: Article
Materias: Q Ciencia > QR Microbiología
R Medicina > RC Medicina Interna, Psiquiatría, Neurología
Divisiones: Medicina
Usuario depositante: Lic. Josimar Pulido
Creadores:
CreadorEmailORCID
Ríos Ibarra, Clara PatriciaNO ESPECIFICADONO ESPECIFICADO
Torres de la Cruz, Víctor ManuelNO ESPECIFICADONO ESPECIFICADO
Ochoa Ruiz, Andrea GabrielaNO ESPECIFICADONO ESPECIFICADO
Rivas Estilla, Ana MaríaNO ESPECIFICADONO ESPECIFICADO
Fecha del depósito: 28 Jun 2019 20:09
Última modificación: 28 Jun 2019 20:09
URI: http://eprints.uanl.mx/id/eprint/16105

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