Virucidal and Synergistic Activity of Polyphenol-Rich Extracts of Seaweeds against Measles Virus

Morán Santibáñez, Karla Selene y Peña Hernández, Mario A. y Cruz Suárez, Lucía Elizabeth y Ricque Marie, Denis y Skouta, Rachid y Vasquez, Abimael H. y Rodríguez Padilla, Cristina y Trejo Ávila, Laura M. (2018) Virucidal and Synergistic Activity of Polyphenol-Rich Extracts of Seaweeds against Measles Virus. Viruses, 10 (9). pp. 1-14. ISSN 1999-4915

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URL o página oficial: http://doi.org/10.3390/v10090465

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Abstract: Although preventable by vaccination, Measles still causes thousands of deaths among young children worldwide. The discovery of new antivirals is a good approach to control new outbreaks that cause such death. In this study, we tested the antiviral activity against Measles virus (MeV)ofPolyphenol-richextracts(PPs)comingfromfiveseaweedscollectedandcultivatedinMexico. AnMTTassaywasperformedtodeterminecytotoxicityeffect,andantiviralactivitywasmeasuredby syncytia reduction assay and confirmed by qPCR. PPs from Ecklonia arborea (formerly Eisenia arborea, Phaeophyceae) and Solieriafiliformis (Rhodophyta) showed the highest Selectivity Index (SI), >3750 and >576.9 respectively. Both PPs extracts were selected to the subsequent experiments owing to their high efficacy and low cytotoxicity compared with ribavirin (SI of 11.57). The combinational effect of PPs with sulphated polysaccharides (SPs) and ribavirin were calculated by using Compusyn software. SynergisticactivitywasobservedbycombiningbothPPswithlowconcentrationsofSolieria filiformis SPs (0.01 µg/mL). The antiviral activity of the best combinations was confirmed by qPCR. Virucidal assay, time of addition, and viral penetration evaluations suggested that PPs act mainly by inactivating the viral particle. To our knowledge, this is the first report of the virucidal effect of Polyphenol-rich extracts of seaweeds.

Tipo de elemento: Article
Materias: Q Ciencia > QH Historia Natural, Biología
Q Ciencia > QR Microbiología
Divisiones: Ciencias Biológicas
Usuario depositante: Lic. Josimar Pulido
Creadores:
CreadorEmailORCID
Morán Santibáñez, Karla SeleneNO ESPECIFICADONO ESPECIFICADO
Peña Hernández, Mario A.NO ESPECIFICADONO ESPECIFICADO
Cruz Suárez, Lucía Elizabethlucia.cruzsr@uanl.edu.mxNO ESPECIFICADO
Ricque Marie, Denisdenis.ricquemr@uanl.edu.mxNO ESPECIFICADO
Skouta, RachidNO ESPECIFICADONO ESPECIFICADO
Vasquez, Abimael H.NO ESPECIFICADONO ESPECIFICADO
Rodríguez Padilla, CristinaNO ESPECIFICADONO ESPECIFICADO
Trejo Ávila, Laura M.NO ESPECIFICADONO ESPECIFICADO
Fecha del depósito: 27 Jun 2019 20:03
Última modificación: 27 Jun 2019 20:03
URI: http://eprints.uanl.mx/id/eprint/16108

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