Maternal overnutrition by hypercaloric diets programs hypothalamic mitochondrial fusion and metabolic dysfunction in rat male offspring

Cárdenas Pérez, Robbi Elizabeth y Fuentes Mera, Lizeth y De la Garza, Ana Laura y Torre Villalvazo, Iván y Reyes Castro, Luis A. y Rodríguez Rocha, Humberto y García García, Aracely y Corona Castillo, Juan Carlos y Tovar, Armando R. y Zambrano, Elena y Ortiz López, Rocío y Saville, Jennifer y Fuller, Maria y Camacho Morales, Alberto (2018) Maternal overnutrition by hypercaloric diets programs hypothalamic mitochondrial fusion and metabolic dysfunction in rat male offspring. Nutrition & Metabolism, 15 (1). pp. 1-16. ISSN 1743-7075

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URL o página oficial: http://doi.org/10.1186/s12986-018-0279-6

Resumen

Abstract Background: Maternal overnutrition including pre-pregnancy, pregnancy and lactation promotes a lipotoxic insult leading to metabolic dysfunction in offspring. Diet-induced obesity models (DIO) show that changes in hypothalamic mitochondria fusion and fission dynamics modulate metabolic dysfunction. Using three selective diet formula including a High fat diet (HFD), Cafeteria (CAF) and High Sugar Diet (HSD), we hypothesized that maternal diets exposure program leads to selective changes in hypothalamic mitochondria fusion and fission dynamics in male offspring leading to metabolic dysfunction which is exacerbated by a second exposure after weaning. Methods: We exposed female Wistar rats to nutritional programming including Chow, HFD, CAF, or HSD for 9 weeks (pre-mating, mating, pregnancy and lactation) or to the same diets to offspring after weaning. We determined body weight, food intake and metabolic parameters in the offspring from 21 to 60 days old. Hypothalamus was dissected at 60 days old to determine mitochondria-ER interaction markers by mRNA expression and western blot and morphology by transmission electron microscopy (TEM). Mitochondrial-ER function was analyzed by confocal microscopy using hypothalamic cell line mHypoA-CLU192. Results: Maternal programming by HFD and CAF leads to failure in glucose, leptin and insulin sensitivity and fat accumulation. Additionally, HFD and CAF programming promote mitochondrial fusion by increasing the expression of MFN2 and decreasing DRP1, respectively. Further, TEM analysis confirms that CAF exposure after programing leads to an increase in mitochondria fusion and enhanced mitochondrial-ER interaction, which partially correlates with metabolic dysfunction and fat accumulation in the HFD and CAF groups. Finally, we identified that lipotoxic palmitic acid stimulus in hypothalamic cells increases Ca2+ overload into mitochondria matrix leading to mitochondrial dysfunction. Conclusions: We concluded that maternal programming by HFD induces hypothalamic mitochondria fusion, metabolic dysfunction and fat accumulation in male offspring, which is exacerbated by HFD or CAF exposure after weaning, potentially due to mitochondria calcium overflux.

Tipo de elemento:

Article

Palabras claves no controlados:

Maternal overnutrition, Diet induced obesity (DIO), Hypothalamus, Mitochondria, Mitochondria dynamics, Fusion, Fission

Materias:

Q Ciencia > QP Fisiología

Divisiones:

Medicina

Usuario depositante:

Lic. Josimar Pulido

Creadores:

Creador	Email	ORCID
Cárdenas Pérez, Robbi Elizabeth	NO ESPECIFICADO	NO ESPECIFICADO
Fuentes Mera, Lizeth	NO ESPECIFICADO	NO ESPECIFICADO
De la Garza, Ana Laura	NO ESPECIFICADO	orcid.org/0000-0002-6049-903X
Torre Villalvazo, Iván	NO ESPECIFICADO	NO ESPECIFICADO
Reyes Castro, Luis A.	NO ESPECIFICADO	NO ESPECIFICADO
Rodríguez Rocha, Humberto	NO ESPECIFICADO	NO ESPECIFICADO
García García, Aracely	NO ESPECIFICADO	NO ESPECIFICADO
Corona Castillo, Juan Carlos	NO ESPECIFICADO	NO ESPECIFICADO
Tovar, Armando R.	NO ESPECIFICADO	NO ESPECIFICADO
Zambrano, Elena	NO ESPECIFICADO	NO ESPECIFICADO
Ortiz López, Rocío	NO ESPECIFICADO	NO ESPECIFICADO
Saville, Jennifer	NO ESPECIFICADO	NO ESPECIFICADO
Fuller, Maria	NO ESPECIFICADO	NO ESPECIFICADO
Camacho Morales, Alberto	NO ESPECIFICADO	orcid.org/0000-0002-2588-9489