Schwann Cell Precursor Transplant in a Rat Spinal Cord Injury Model

De la Garza Castro, Oscar y Martínez Rodríguez, Herminia Guadalupe y Sánchez González, Sandra Gabriela y Vidal Torres, Oscar y Arreola Romero, Azalea y De La Garza Pineda, Oscar y Ancer Arellano, Adriana Guadalupe y Guzmán López, Santos y Elizondo Omaña, Rodrigo Enrique (2018) Schwann Cell Precursor Transplant in a Rat Spinal Cord Injury Model. Revista de investigación Clínica, 70 (2). pp. 88-95. ISSN 0034-8376

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URL o página oficial: http://doi.org/10.24875/RIC.18002466

Resumen

Background: Differentiation of mesenchymal stem cells into Schwann cell precursors could reverse established lesions and sequelae of medullary transection. Objective: The objective of this study was to study the clinical response of mesenchymal stem cell transplantation with Schwann precursor cell transplantation in a rat spinal cord injury model, using motor function and histopathologic studies. Materials and methods: A total of 28 Sprague-Dawley rats were randomly divided among four groups (n = 7 in each): sham group, control group, mesenchymal stem cell transplant group, and Schwann cell precursor transplant group. The surgical procedure was a laminectomy with transection of the spinal cord at the T11 level in the transplant groups and the injury control group. After 1 week, the transplant groups received stem cells directly in the injury site. Hind limb motor function was assessed using the locomotive scale of Basso, Beattie, and Bresnahan. 1 month after transplantation, all specimens were sacrificed to make a histopathologic description of sections taken from the site of injury and where stem cells were transplanted. Mean scores of mobility were compared using analysis of variance (ANOVA) of one factor with 95% reliability between groups and ANOVA of repetitive measures to evaluate evolution in the same group. Results: We observed that the control group had statistically greater mobility than the other groups (p < 0.0001) and that the group with spinal injury without treatment had the lowest mean mobility. The mobility score values from the Schwann cell precursor group were statistically higher than the group treated with mesenchymal stem cells (p < 0.0001). Conclusion: Schwann precursor cells had a greater effect on locomotive function than mesenchymal stem cells.

Tipo de elemento: Article
Materias: R Medicina > RC Medicina Interna, Psiquiatría, Neurología
Divisiones: Medicina
Usuario depositante: Lic. Josimar Pulido
Creadores:
CreadorEmailORCID
De la Garza Castro, OscarNO ESPECIFICADONO ESPECIFICADO
Martínez Rodríguez, Herminia GuadalupeNO ESPECIFICADONO ESPECIFICADO
Sánchez González, Sandra GabrielaNO ESPECIFICADONO ESPECIFICADO
Vidal Torres, OscarNO ESPECIFICADONO ESPECIFICADO
Arreola Romero, AzaleaNO ESPECIFICADONO ESPECIFICADO
De La Garza Pineda, OscarNO ESPECIFICADONO ESPECIFICADO
Ancer Arellano, Adriana GuadalupeNO ESPECIFICADONO ESPECIFICADO
Guzmán López, SantosNO ESPECIFICADONO ESPECIFICADO
Elizondo Omaña, Rodrigo EnriqueNO ESPECIFICADONO ESPECIFICADO
Fecha del depósito: 25 Mayo 2020 12:57
Última modificación: 28 Oct 2020 15:26
URI: http://eprints.uanl.mx/id/eprint/16318

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