Exploring the contribution of dietary protein from poultry by product meal and fish meal to the growth of catfish Ictalurus punctatus by means of nitrogen stable isotopes

Garcia, Oscar y Cruz, Julio y Ramirez, Carlos y Villarreal, David y Gamboa, Julian (2018) Exploring the contribution of dietary protein from poultry by product meal and fish meal to the growth of catfish Ictalurus punctatus by means of nitrogen stable isotopes. Latin American Journal of Aquatic Research, 46 (1). pp. 37-44. ISSN 0718560X

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BACKGROUND Mycobacterium tuberculosis is an intracellular pathogen, which may either block cellular defensive mechanisms and survive inside the host cell or induce cell death. Several studies are still exploring the mechanisms involved in these processes. OBJECTIVES To evaluate the genomic instability of M. tuberculosis-infected macrophages and compare it with that of uninfected macrophages. METHODS We analysed the possible variations in the genomic instability of Mycobacterium-infected macrophages using the DNA breakage detection fluorescence in situ hybridisation (DBD-FISH) technique with a whole human genome DNA probe. FINDINGS Quantitative image analyses showed a significant increase in DNA damage in infected macrophages as compared with uninfected cells. DNA breaks were localised in nuclear membrane blebs, as confirmed with DNA fragmentation assay. Furthermore, a significant increase in micronuclei and nuclear abnormalities were observed in infected macrophages versus uninfected cells. MAIN CONCLUSIONS Genomic instability occurs during mycobacterial infection and these data may be seminal for future research on host cell DNA damage in M. tuberculosis infection.

Tipo de elemento: Article
Divisiones: Ciencias Biológicas
Usuario depositante: Lic. Josimar Pulido
Creadores:
CreadorEmailORCID
Garcia, OscarNO ESPECIFICADONO ESPECIFICADO
Cruz, JulioNO ESPECIFICADONO ESPECIFICADO
Ramirez, CarlosNO ESPECIFICADONO ESPECIFICADO
Villarreal, DavidNO ESPECIFICADONO ESPECIFICADO
Gamboa, JulianNO ESPECIFICADONO ESPECIFICADO
Fecha del depósito: 20 Ago 2019 20:53
Última modificación: 20 Ago 2019 20:53
URI: http://eprints.uanl.mx/id/eprint/16319

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