Mycobacterium tuberculosis promotes genomic instability in macrophages

Castro Garza, Jorge Enrique y Luévano Martínez, Miriam Lorena y Villarreal Treviño, Licet y Gosálvez, Jaime y Fernández, José Luis y Dávila Rodríguez, Martha Imelda y García Vielma, Catalina y González Hernández, Silvia y Cortés Gutiérrez, Elva Irene (2018) Mycobacterium tuberculosis promotes genomic instability in macrophages. Memórias do Instituto Oswaldo Cruz, 113 (3). pp. 161-166. ISSN 0074-0276

Vista previa

Texto 170.pdf - Versión Publicada Available under License Creative Commons Attribution Non-commercial No Derivatives. Download (811kB) | Vista previa

Resumen

BACKGROUND Mycobacterium tuberculosis is an intracellular pathogen, which may either block cellular defensive mechanisms and survive inside the host cell or induce cell death. Several studies are still exploring the mechanisms involved in these processes. OBJECTIVES To evaluate the genomic instability of M. tuberculosis-infected macrophages and compare it with that of uninfected macrophages. METHODS We analysed the possible variations in the genomic instability of Mycobacterium-infected macrophages using the DNA breakage detection fluorescence in situ hybridisation (DBD-FISH) technique with a whole human genome DNA probe. FINDINGS Quantitative image analyses showed a significant increase in DNA damage in infected macrophages as compared with uninfected cells. DNA breaks were localised in nuclear membrane blebs, as confirmed with DNA fragmentation assay. Furthermore, a significant increase in micronuclei and nuclear abnormalities were observed in infected macrophages versus uninfected cells. MAIN CONCLUSIONS Genomic instability occurs during mycobacterial infection and these data may be seminal for future research on host cell DNA damage in M. tuberculosis infection. Key words: Mycobacterium tuberculosis - alkaline-labile sites - DNA breakage detection fluorescence in situ hybridisation (DBD-FISH)

Actions (login required)

Ver elemento