Mycobacterium tuberculosis promotes genomic instability in macrophages

Castro Garza, Jorge Enrique y Luévano Martínez, Miriam Lorena y Villarreal Treviño, Licet y Gosálvez, Jaime y Fernández, José Luis y Dávila Rodríguez, Martha Imelda y García Vielma, Catalina y González Hernández, Silvia y Cortes Gutiérrez, Elva Irene (2018) Mycobacterium tuberculosis promotes genomic instability in macrophages. Memórias do Instituto Oswaldo Cruz, 113 (3). pp. 161-166. ISSN 0074-0276

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Resumen

BACKGROUND Mycobacterium tuberculosis is an intracellular pathogen, which may either block cellular defensive mechanisms and survive inside the host cell or induce cell death. Several studies are still exploring the mechanisms involved in these processes. OBJECTIVES To evaluate the genomic instability of M. tuberculosis-infected macrophages and compare it with that of uninfected macrophages. METHODS We analysed the possible variations in the genomic instability of Mycobacterium-infected macrophages using the DNA breakage detection fluorescence in situ hybridisation (DBD-FISH) technique with a whole human genome DNA probe. FINDINGS Quantitative image analyses showed a significant increase in DNA damage in infected macrophages as compared with uninfected cells. DNA breaks were localised in nuclear membrane blebs, as confirmed with DNA fragmentation assay. Furthermore, a significant increase in micronuclei and nuclear abnormalities were observed in infected macrophages versus uninfected cells. MAIN CONCLUSIONS Genomic instability occurs during mycobacterial infection and these data may be seminal for future research on host cell DNA damage in M. tuberculosis infection. Key words: Mycobacterium tuberculosis - alkaline-labile sites - DNA breakage detection fluorescence in situ hybridisation (DBD-FISH)

Tipo de elemento: Article
Divisiones: Ciencias Biológicas
Usuario depositante: Lic. Josimar Pulido
Creadores:
CreadorEmailORCID
Castro Garza, Jorge EnriqueNO ESPECIFICADONO ESPECIFICADO
Luévano Martínez, Miriam LorenaNO ESPECIFICADONO ESPECIFICADO
Villarreal Treviño, LicetNO ESPECIFICADONO ESPECIFICADO
Gosálvez, JaimeNO ESPECIFICADONO ESPECIFICADO
Fernández, José LuisNO ESPECIFICADONO ESPECIFICADO
Dávila Rodríguez, Martha ImeldaNO ESPECIFICADONO ESPECIFICADO
García Vielma, CatalinaNO ESPECIFICADONO ESPECIFICADO
González Hernández, SilviaNO ESPECIFICADONO ESPECIFICADO
Cortes Gutiérrez, Elva IreneNO ESPECIFICADONO ESPECIFICADO
Fecha del depósito: 31 Jul 2019 21:03
Última modificación: 12 Ago 2019 14:43
URI: http://eprints.uanl.mx/id/eprint/16325

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