Molecular cloning of the myo-inositol oxygenase gene from the kidney of baboons

González Álvarez, Rafael y Pérez Ibave, Diana Cristina y Garza Rodríguez, María de Lourdes y Lugo Trampe, Ángel y Delgado Enciso, Iván y Tejero Barrera, María Elizabeth y Martínez de Villarreal, Laura Elia y Garza Guajardo, Raquel y Sánchez Chaparro, María Marisela y Ruiz Aymá, Gabriel y Barboza Quintana, Oralia y Barrera Saldaña, Hugo Alberto y Rocha Pizaña, María del Refugio y Rodríguez Sánchez, Irám Pablo (2017) Molecular cloning of the myo-inositol oxygenase gene from the kidney of baboons. Biomedical reports, 7 (4). pp. 301-305. ISSN 2049-9434

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Resumen

Abstract. The enzyme myo-Inositol oxygenase (MIOX) is also termed ALDRL6. It is a kidney‑specific member of the aldo‑keto reductase family. MIOX catalyzes the first reaction involved in the myo‑inositol metabolism signaling pathway and is fully expressed in mammalian tissues. MIOX catalyzes the oxidative cleavage of myo‑Inositol and its epimer, D-chiro-Inositol to D-glucuronate. The dioxygen-dependent cleavage of the C6 and C1 bond in myo‑Inositol is achieved by utilizing the Fe2+/Fe3+ binuclear iron center of MIOX. This enzyme has also been implicated in the complications of diabetes, including diabetic nephropathy. The MIOX gene was amplified with reverse transcription‑polymerase chain reaction from baboon tissue samples, and the product was cloned and sequenced. MIOX expression in the baboon kidney is described in the present study. The percentages of nucleotide and amino acid similarities between baboons and humans were 95 and 96%, respectively. The MIOX protein of the baboon may be structurally identical to that of humans. Furthermore, the evolutionary changes, which have affected these sequences, have resulted from purifying forces.

Tipo de elemento:	Article
Materias:	Q Ciencia > QH Historia Natural, Biología
Divisiones:	Ciencias Biológicas
Usuario depositante:	Editor Repositorio
Creadores:	Creador Email ORCID González Álvarez, Rafael NO ESPECIFICADO NO ESPECIFICADO Pérez Ibave, Diana Cristina NO ESPECIFICADO NO ESPECIFICADO Garza Rodríguez, María de Lourdes lulugarza8@gmail.com orcid.org/0000-0001-7096-2713 Lugo Trampe, Ángel NO ESPECIFICADO NO ESPECIFICADO Delgado Enciso, Iván NO ESPECIFICADO NO ESPECIFICADO Tejero Barrera, María Elizabeth NO ESPECIFICADO NO ESPECIFICADO Martínez de Villarreal, Laura Elia NO ESPECIFICADO NO ESPECIFICADO Garza Guajardo, Raquel NO ESPECIFICADO NO ESPECIFICADO Sánchez Chaparro, María Marisela NO ESPECIFICADO NO ESPECIFICADO Ruiz Aymá, Gabriel gabriel.ruizym@uanl.edu.mx NO ESPECIFICADO Barboza Quintana, Oralia NO ESPECIFICADO NO ESPECIFICADO Barrera Saldaña, Hugo Alberto NO ESPECIFICADO NO ESPECIFICADO Rocha Pizaña, María del Refugio NO ESPECIFICADO NO ESPECIFICADO Rodríguez Sánchez, Irám Pablo NO ESPECIFICADO NO ESPECIFICADO
Fecha del depósito:	29 Abr 2020 15:00
Última modificación:	05 Mar 2024 15:41
URI:	http://eprints.uanl.mx/id/eprint/17530

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