Detection of anti-HspX antibodies and HspX protein in patient sera for the identification of recent latent infection by Mycobacterium tuberculosis

Izzo, Angelo A. y Castro Garza, Jorge y García Jacobo, Paola Janeth y Rivera Morales, Lydia Guadalupe y Quinn, Frederick D. y Barber, James y Karls, Russell y Haas, Debra y Helms, Shelly y Gupta, Tuhina y Blumberg, Henry y Tapia, Jane y Luna Cruz, Itza Eloísa y Rendón, Adrián y Vargas Villarreal, Javier y Vera Cabrera, Lucio y Rodríguez Padilla, Cristina (2017) Detection of anti-HspX antibodies and HspX protein in patient sera for the identification of recent latent infection by Mycobacterium tuberculosis. PLOS ONE, 12 (8). pp. 1-13. ISSN 1932-6203

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Abstract Mycobacterium tuberculosis is a pathogen causing tuberculosis (TB) a spectrum of disease including acute and asymptomatic latent stages. Identifying and treating latently-infected patients constitutes one of the most important impediments to TB control efforts. Those individuals can remain undiagnosed for decades serving as potential reservoirs for disease reactivation. Tests for the accurate diagnosis of latent infection currently are unavailable. HspX protein (α-crystallin), encoded by Rv2031c gene, is produced in vitro by M. tuberculosis during stationary growth phase and hypoxic or acidic culture conditions. In this study, using standard, and Luminex xMAP® bead capture ELISA, respectively, we report on detection of anti-HspX IgG and IgM antibodies and HspX protein in sera from acute and latent TB patients. For the antibody screen, levels of IgG and IgM antibodies were similar between non-infected and active TB patients; however, individuals classified into the group with latent TB showed higher values of anti-HspX IgM (p = 0.003) compared to active TB patients. Using the bead capture antigen detection assay, HspX protein was detected in sera from 56.5% of putative latent cases (p< 0.050) compared to the background median with an average of 9,900 pg/ml and a range of 1,000 to 36,000 pg/ml. Thus, presence of anti-HspX IgM antibodies and HspX protein in sera may be markers of latent TB.

Tipo de elemento: Article
Materias: Q Ciencia > QR Microbiología
Divisiones: Ciencias Biológicas
Usuario depositante: Lic. Jesús E. Alvarado
Creadores:
CreadorEmailORCID
Izzo, Angelo A.NO ESPECIFICADONO ESPECIFICADO
Castro Garza, JorgeNO ESPECIFICADONO ESPECIFICADO
García Jacobo, Paola JanethNO ESPECIFICADONO ESPECIFICADO
Rivera Morales, Lydia GuadalupeNO ESPECIFICADONO ESPECIFICADO
Quinn, Frederick D.NO ESPECIFICADONO ESPECIFICADO
Barber, JamesNO ESPECIFICADONO ESPECIFICADO
Karls, RussellNO ESPECIFICADONO ESPECIFICADO
Haas, DebraNO ESPECIFICADONO ESPECIFICADO
Helms, ShellyNO ESPECIFICADONO ESPECIFICADO
Gupta, TuhinaNO ESPECIFICADONO ESPECIFICADO
Blumberg, HenryNO ESPECIFICADONO ESPECIFICADO
Tapia, JaneNO ESPECIFICADONO ESPECIFICADO
Luna Cruz, Itza EloísaNO ESPECIFICADONO ESPECIFICADO
Rendón, AdriánNO ESPECIFICADONO ESPECIFICADO
Vargas Villarreal, JavierNO ESPECIFICADONO ESPECIFICADO
Vera Cabrera, LucioNO ESPECIFICADONO ESPECIFICADO
Rodríguez Padilla, CristinaNO ESPECIFICADONO ESPECIFICADO
Fecha del depósito: 12 Mar 2020 18:43
Última modificación: 05 Jun 2020 14:50
URI: http://eprints.uanl.mx/id/eprint/18182

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