Persistence of Toxic Activity of Fermentation Extracts from Bacillus thuringiensis var. israelensis after More Than Three Decades of Storage

Galán Wong, Luis Jesús y Gamiño Hernández, Rossana y Fernández Chapa, David y García Díaz, Graciela y De la Garza Ramos, Myriam Angélica y Guajardo Barbosa, Claudio y Luna Olvera, Hugo Alberto (2017) Persistence of Toxic Activity of Fermentation Extracts from Bacillus thuringiensis var. israelensis after More Than Three Decades of Storage. International Journal of Microbiology, 2017. pp. 1-6. ISSN 1687-918X

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URL o página oficial: http://doi.org/10.1155/2017/5402748

Resumen

This study was carried out to determine the persistence of toxicity of fermentation extracts of Bacillus thuringiensis var. israelensis after more than three decades of storage. For this purpose, a population of Aedes aegypti was established. The mortality rate of 20 spore-crystal extracts purified using the acetone-lactose coprecipitation method was measured and evaluated by bioassays according to a modified WHO protocol. The extracts with the highest mortality rate were determined in triplicate by their LD50 and LD98. All extracts showed toxicity at the highest tested dose (1000 ppm) and some, such as strains 3260 and 3501, still killed larvae at doses as low as 0.01 ppm. These data are surprising because no study on the activity of B. thuringiensis toxic proteins after such a long storage time has been reported.

Tipo de elemento: Article
Materias: Q Ciencia > QR Microbiología
Divisiones: Ciencias Biológicas
Usuario depositante: Editor Repositorio
Creadores:
CreadorEmailORCID
Galán Wong, Luis JesúsNO ESPECIFICADONO ESPECIFICADO
Gamiño Hernández, RossanaNO ESPECIFICADONO ESPECIFICADO
Fernández Chapa, DavidNO ESPECIFICADONO ESPECIFICADO
García Díaz, GracielaNO ESPECIFICADONO ESPECIFICADO
De la Garza Ramos, Myriam AngélicaNO ESPECIFICADONO ESPECIFICADO
Guajardo Barbosa, ClaudioNO ESPECIFICADONO ESPECIFICADO
Luna Olvera, Hugo AlbertoNO ESPECIFICADONO ESPECIFICADO
Fecha del depósito: 11 Mar 2020 15:46
Última modificación: 08 Mayo 2020 16:03
URI: http://eprints.uanl.mx/id/eprint/18205

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