Biodegradable nanoparticles loaded with tetrameric melittin: preparation and membrane disruption evaluation

González Horta, Azucena y Matamoros Acosta, Arely y Chávez Montes, Abelardo y Castro Ríos, Rocio y Lara Arias, Jorge (2017) Biodegradable nanoparticles loaded with tetrameric melittin: preparation and membrane disruption evaluation. General physiology and biophysics, 36 (04). pp. 373-381. ISSN 1338-4325

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URL o página oficial: http://doi.org/10.4149/gpb_2017011

Resumen

Abstract. Melittin is the main component of bee venom consisting of 26 amino acids that has multiple effects, including antibacterial, antiviral and anti-inflammatory in various cell types. This peptide forms pores in biological membranes and triggers cell death. Therefore it has potential as an anticancer therapy. However, the therapeutic application of melittin is limited due to its main side effect, hemolysis, which is especially pronounced following intravenous administration. In the present study, we formulated tetrameric melittin-carrying poly-D,L-lactic-co-glycolic acid nanoparticles (PLGANPs) and analyzed the lytic activity of this system on liposomes that resembles breast cancer cells. Tetrameric melittin binds avidly to PLGA-NPs with an encapsulation efficiency of 97% and retains its lytic activity demonstrating the effectiveness of PLGA-NPs as nanocarriers for this cytolytic peptide. Key words: Melittin — PLGA nanoparticles — Liposomes — Leakage — Fluorescence — Circular dichroism

Tipo de elemento: Article
Palabras claves no controlados: PLGA nanoparticles — Liposomes — Leakage — Fluorescence — Circular dichroism
Materias: Q Ciencia > QH Historia Natural, Biología
Divisiones: Ciencias Biológicas
Usuario depositante: Editor Repositorio
Creadores:
CreadorEmailORCID
González Horta, AzucenaNO ESPECIFICADONO ESPECIFICADO
Matamoros Acosta, ArelyNO ESPECIFICADONO ESPECIFICADO
Chávez Montes, AbelardoNO ESPECIFICADONO ESPECIFICADO
Castro Ríos, RocioNO ESPECIFICADONO ESPECIFICADO
Lara Arias, JorgeNO ESPECIFICADONO ESPECIFICADO
Fecha del depósito: 10 Mar 2020 18:49
Última modificación: 10 Mar 2020 18:49
URI: http://eprints.uanl.mx/id/eprint/18214

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