Multicenter Study of Method-Dependent Epidemiological Cutoff Values for Detection of Resistance in Candida spp. and Aspergillus spp. to Amphotericin B and Echinocandins for the Etest Agar Diffusion Method

Espinel Ingroff, A. y Arendrup, M. y Cantón, E. y Córdoba, S. y Dannaoui, E. y García Rodríguez, J. y González, Gloria M. y Govender, N. P. y Martin Mazuelos, E. y Lackner, Michaela y Lass Flörl, Cornelia y Linares Sicilia, M. J. y Rodríguez Iglesias, M. A. y Pelaez, T. y Shields, R. K. y Garcia Effron, G. y Guinea, J. y Sanguinetti, M. y Turnidge, J. (2016) Multicenter Study of Method-Dependent Epidemiological Cutoff Values for Detection of Resistance in Candida spp. and Aspergillus spp. to Amphotericin B and Echinocandins for the Etest Agar Diffusion Method. Antimicrobial Agents and Chemotherapy, 61 (1). pp. 1-10. ISSN 0066-4804

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URL o página oficial: http://doi.org/10.1128/AAC.01792-16

Resumen

BSTRACT Method-dependent Etest epidemiological cutoff values (ECVs) are not available for susceptibility testing of either Candida or Aspergillus species with amphotericin B or echinocandins. In addition, reference caspofungin MICs for Candida spp. are unreliable. Candida and Aspergillus species wild-type (WT) Etest MIC distributions (microorganisms in a species-drug combination with no detectable phenotypic resistance) were established for 4,341 Candida albicans, 113 C. dubliniensis, 1,683 C. glabrata species complex (SC), 709 C. krusei, 767 C. parapsilosis SC, 796 C. tropicalis, 1,637 Aspergillus fumigatus SC, 238 A. flavus SC, 321 A. niger SC, and 247 A. terreus SC isolates. Etest MICs from 15 laboratories (in Argentina, Europe, Mexico, South Africa, and the United States) were pooled to establish Etest ECVs. Anidulafungin, caspofungin, micafungin, and amphotericin B ECVs (in micrograms per milliliter) encompassing �97.5% of the statistically modeled population were 0.016, 0.5, 0.03, and 1 for C. albicans; 0.03, 1, 0.03, and 2 for C. glabrata SC; 0.06, 1, 0.25, and 4 for C. krusei; 8, 4, 2, and 2 forC. parapsilosis SC; and 0.03, 1, 0.12, and 2 for C. tropicalis. The amphotericin B ECV was 0.25 � g/ml for C. dubliniensis and 2, 8, 2, and 16 � g/ml for the complexes of A. fumigatus, A. flavus, A. niger, and A. terreus, respectively. While anidulafung in Etest ECVs classified 92% of the Candida fks mutants evaluated as non-WT, the performance was lower for caspofungin (75%) and micafungin (84%) cutoffs. Finally, although anidulafungin (as an echinocandin surrogate susceptibility marker) and amphotericin B ECVs should identify Candida and Aspergillus isolates with reduced susceptibility to these agents using the Etest, these ECVs will not categorize a fungal isolate as susceptible or resistant, as breakpoints do. KEYWORDS ECVs, Etest ECVs, Etest MICs Candida, Etest MICs Aspergillus, WT isolates, amphotericin B resistance, antifungal resistance, echinocandin resistance, non-WT, susceptibility marker

Tipo de elemento: Article
Materias: R Medicina > R Medicina en General
Divisiones: Medicina
Usuario depositante: Editor Repositorio
Creadores:
CreadorEmailORCID
Espinel Ingroff, A.NO ESPECIFICADONO ESPECIFICADO
Arendrup, M.NO ESPECIFICADONO ESPECIFICADO
Cantón, E.NO ESPECIFICADONO ESPECIFICADO
Córdoba, S.NO ESPECIFICADONO ESPECIFICADO
Dannaoui, E.NO ESPECIFICADONO ESPECIFICADO
García Rodríguez, J.NO ESPECIFICADONO ESPECIFICADO
González, Gloria M.NO ESPECIFICADONO ESPECIFICADO
Govender, N. P.NO ESPECIFICADONO ESPECIFICADO
Martin Mazuelos, E.NO ESPECIFICADONO ESPECIFICADO
Lackner, MichaelaNO ESPECIFICADONO ESPECIFICADO
Lass Flörl, CorneliaNO ESPECIFICADONO ESPECIFICADO
Linares Sicilia, M. J.NO ESPECIFICADONO ESPECIFICADO
Rodríguez Iglesias, M. A.NO ESPECIFICADONO ESPECIFICADO
Pelaez, T.NO ESPECIFICADONO ESPECIFICADO
Shields, R. K.NO ESPECIFICADONO ESPECIFICADO
Garcia Effron, G.NO ESPECIFICADONO ESPECIFICADO
Guinea, J.NO ESPECIFICADONO ESPECIFICADO
Sanguinetti, M.NO ESPECIFICADONO ESPECIFICADO
Turnidge, J.NO ESPECIFICADONO ESPECIFICADO
Fecha del depósito: 26 Feb 2020 16:57
Última modificación: 21 Mayo 2020 18:48
URI: http://eprints.uanl.mx/id/eprint/18265

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