Multiplex PCR for simultaneous genotyping of kdr mutations V410L, V1016I and F1534C in Aedes aegypti (L.)

Villanueva Segura, Karina y Ponce García, Gustavo y López Monroy, Beatriz y Mora Jasso, Esteban de Jesús y Perales, Lucia y González Santillán, Francisco Javier y Ontiveros Zapata, Kevin Aarón y Dávila Barboza, Jesús Antonio y Flores, Adriana E. (2020) Multiplex PCR for simultaneous genotyping of kdr mutations V410L, V1016I and F1534C in Aedes aegypti (L.). Parasites & Vectors, 13 (1). pp. 1-8. ISSN 1756-3305

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Abstract Background Knockdown resistance (kdr) is the main mechanism that confers resistance to pyrethroids and DDT. This is a product of non-synonymous mutations in the voltage-gated sodium channel (vgsc) gene, and these mutations produce a change of a single amino acid which reduces the affinity of the target site for the insecticide molecule. In Mexico, V410L, V1016I and F1534C mutations are common in pyrethroid-resistant Aedes aegypti (L.) populations. Methods A multiplex PCR was developed to detect the V410L, V1016I and F1534C mutations in Ae. aegypti. The validation of the technique was carried out by DNA sequencing using field populations previously characterized for the three mutations through allele-specific PCR (AS-PCR) and with different levels of genotypic frequencies. Results The standardized protocol for multiplex end-point PCR was highly effective in detecting 15 genotypes considering the three mutations V410L, V1106I and F1534C, in 12 field populations of Ae. aegypti from Mexico. A complete concordance with AS-PCR and DNA sequencing was found for the simultaneous detection of the three kdr mutations. Conclusions Our diagnostic method is highly effective for the simultaneous detection of V410L, V1016I and F1534C, when they co-occur. This technique represents a viable alternative to complement and strengthen current monitoring and resistance management strategies against Ae. aegypti.

Tipo de elemento: Article
Palabras claves no controlados: Multiplex PCR, Aedes aegypti, kdr, V410L, V1016I, F1534C
Materias: Q Ciencia > QL Zoología
Divisiones: Ciencias Biológicas
Usuario depositante: Editor Repositorio
Creadores:
CreadorEmailORCID
Villanueva Segura, KarinaNO ESPECIFICADONO ESPECIFICADO
Ponce García, GustavoNO ESPECIFICADONO ESPECIFICADO
López Monroy, BeatrizNO ESPECIFICADONO ESPECIFICADO
Mora Jasso, Esteban de JesúsNO ESPECIFICADONO ESPECIFICADO
Perales, LuciaNO ESPECIFICADONO ESPECIFICADO
González Santillán, Francisco JavierNO ESPECIFICADONO ESPECIFICADO
Ontiveros Zapata, Kevin AarónNO ESPECIFICADONO ESPECIFICADO
Dávila Barboza, Jesús AntonioNO ESPECIFICADONO ESPECIFICADO
Flores, Adriana E.NO ESPECIFICADONO ESPECIFICADO
Fecha del depósito: 25 Mayo 2022 20:13
Última modificación: 05 Mar 2024 18:57
URI: http://eprints.uanl.mx/id/eprint/23292

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