Cetylpyridinium chloride inhibits human breast tumor cells growth in a no-selective way

García Cuellar, Claudia María y Hernández Delgadillo, René y Solís Soto, Juan Manuel y Meester, Irene y Sánchez Pérez, Yesennia y Nakagoshi Cepeda, Sergio Eduardo y Nakagoshi Cepeda, María Argelia Akemi y Chellam, Shankararaman y Cabral Romero, Claudio (2022) Cetylpyridinium chloride inhibits human breast tumor cells growth in a no-selective way. Journal of Applied Biomaterials & Functional Materials, 20. pp. 1-9. ISSN 2280-8000

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Resumen

Objective: Analyze the antitumor capacity of cetylpyridinium chloride (CPC) on human breast tumor cells, and the possible action mechanism. Material and methods: The human breast tumor cells MCF-7 and no-tumor breast cells MCF-10A were exposed to CPC under various condition (concentration and duration). Cell viability was measured with MTT assay, the LIVE/DEAD assay, and fluorescence microscopy. Membrane permeability after CPC exposure was evaluated by Calcein AM assay, mitochondrial morphology with a MitoView staining, and genotoxicity with the comet assay and fluorescence microscopy. Results: CPC was cytotoxic to both MCF-7 and MCF-10A as of a 24-h exposure to 0.1 µM. Cytotoxicity was dose-dependent and reached 91% for MCF-7 and 78% for MCF-10A after a 24-h exposure to 100 µM CPC, which outperformed the positive control doxorubicin in effectiveness and selectivity. The LD50 of CPC on was 6 µM for MCF-7 and 8 µM for MCF-10A, yielding a selectivity index of 1.41. A time response analysis revealed 64% dead cells after only 5 min of exposure to 100 µM CPC. With respect to the action mechanisms, the comet assay did not reveal genome fragmentation. On the other hand, membrane damage was dose-dependent and may also affect mitochondrial morphology. Conclusion: Cetylpyridinium chloride inhibits MCF-7 cell growing in a non-selective way as of 5 min of exposure. The action mechanism of CPC on tumor cells involves cell membrane damage without change neither mitochondrial morphology nor genotoxicity.

Tipo de elemento:	Article
Palabras claves no controlados:	Antitumor activity, cetylpyridinium chloride, human breast cancer, chemotherapy, quaternary ammonium salts, LD50 assay
Materias:	R Medicina > RC Medicina Interna, Psiquiatría, Neurología
Divisiones:	Ciencias Biológicas
Usuario depositante:	Editor Repositorio
Creadores:	Creador Email ORCID García Cuellar, Claudia María NO ESPECIFICADO NO ESPECIFICADO Hernández Delgadillo, René NO ESPECIFICADO orcid.org/0000-0001-6799-1943 Solís Soto, Juan Manuel NO ESPECIFICADO NO ESPECIFICADO Meester, Irene NO ESPECIFICADO NO ESPECIFICADO Sánchez Pérez, Yesennia NO ESPECIFICADO NO ESPECIFICADO Nakagoshi Cepeda, Sergio Eduardo NO ESPECIFICADO NO ESPECIFICADO Nakagoshi Cepeda, María Argelia Akemi NO ESPECIFICADO NO ESPECIFICADO Chellam, Shankararaman NO ESPECIFICADO NO ESPECIFICADO Cabral Romero, Claudio NO ESPECIFICADO NO ESPECIFICADO
Fecha del depósito:	25 Mayo 2022 20:15
Última modificación:	25 Mayo 2022 20:15
URI:	http://eprints.uanl.mx/id/eprint/23293

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