Low-Dose Amphotericin B and Murine Dialyzable Spleen Extracts Protect against SystemicCandidaInfection in Mice

Robledo Ávila, F. y Pérez Tapia, M. y Limón Flores, A. y Pavon, L. y Hernández Pando, R. y Wong Baeza, I. y González González, G. y Tovar, C. y Estrada Parra, S. y Estrada García, I. (2013) Low-Dose Amphotericin B and Murine Dialyzable Spleen Extracts Protect against SystemicCandidaInfection in Mice. Clinical and Developmental Immunology, 2013. pp. 1-7. ISSN 1740-2522

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URL o página oficial: http://doi.org/10.1155/2013/194064

Resumen

Candida albicans causes opportunistic systemic infections with high mortality (30%–50%). Despite significant nephrotoxicity, amphotericin (AmB) is still used for the treatment of this serious fungal infection. Therefore, alternative treatments are urgently needed. Dialyzable leukocyte extracts have been used successfully to treat patients with mucocutaneous candidiasis, but their effectiveness in systemic candidiasis has not been evaluated. In this study, low-dose AmB (0.1 mg/kg) plus 10 pg of murine dialyzable spleen extracts (mDSE) were tested in a systemic candidiasis mouse model. Survival, tissue fungal burden, kidney damage, kidney cytokines, and serum levels of IL-6 and hepcidin were evaluated. Our results showed that the combined treatment of low-dose AmB plus mDSE improved survival and reduced kidney fungal burden and histopathology; these effects correlated with increased kidney concentration of IFN-γ and TGF-β1, decreased levels of TNF-α, IL-6, and IL-10, as well as high levels of systemic IL-6 and hepcidin. Low-dose AmB and mDSE synergized to clear the infectious agent and reduced tissue damage, confirming the efficacy of a low dose of AmB, which might decrease the risk of drug toxicity. Further studies are necessary to explore these findings and its implications in future therapeutic approaches.

Tipo de elemento: Article
Materias: Q Ciencia > QR Microbiología
Divisiones: Medicina
Usuario depositante: Editor Repositorio
Creadores:
CreadorEmailORCID
Robledo Ávila, F.NO ESPECIFICADONO ESPECIFICADO
Pérez Tapia, M.NO ESPECIFICADONO ESPECIFICADO
Limón Flores, A.NO ESPECIFICADONO ESPECIFICADO
Pavon, L.NO ESPECIFICADONO ESPECIFICADO
Hernández Pando, R.NO ESPECIFICADONO ESPECIFICADO
Wong Baeza, I.NO ESPECIFICADONO ESPECIFICADO
González González, G.NO ESPECIFICADONO ESPECIFICADO
Tovar, C.NO ESPECIFICADONO ESPECIFICADO
Estrada Parra, S.NO ESPECIFICADONO ESPECIFICADO
Estrada García, I.NO ESPECIFICADONO ESPECIFICADO
Fecha del depósito: 20 Jun 2023 22:16
Última modificación: 20 Jun 2023 22:16
URI: http://eprints.uanl.mx/id/eprint/25594

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