Antimicrobial activity of gimnosperma glutinosum(spreng.) less.(asteraceae) metanol extracts against helicobacter pylori

Gómez Flores, Ricardo Alberto y Espinosa Ramos, David Alonso y Quintanilla Licea, Ramiro y Barragán González, María Porfiria y Tamez Guerra, Patricia y Tamez Guerra, Reyes y Rodríguez Padilla, Cristina (2016) Antimicrobial activity of gimnosperma glutinosum(spreng.) less.(asteraceae) metanol extracts against helicobacter pylori. African Journal of Traditional, Complementary and Alternative Medicines, 13 (4). pp. 55-59. ISSN 0189-6016

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URL o página oficial: http://doi.org/10.21010/ajtcam.v13i4.9

Resumen

Background: Prolonged use of antibiotics may lead to the selection of drug-resistant bacteria; as a result, efforts are being made to identify new and effective antimicrobial agents, particularly, from medicinal plants, against bacterial infections. Antimicrobial activity of Gymnosperma glutinosum against Helicobacter pylori has not yet been reported. Materials and methods: The antibacterial in vitro effect of Gymnosperma glutinosum methanol leaf extracts against Helicobacter pylori (ATCC 43504) was evaluated in liquid medium by the colorimetric 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) reduction assay and in solid medium by the colony forming units (CFU) method. Results: Methanol extracts significantly (p<0.05) inhibited in vitro H. pylori growth in liquid medium from 24% to 82% at concentrations ranging from 31.25 mg/ml to 500 mg/ml, respectively, and in solid medium the extracts caused significant (p<0.05) 52% and 100% bacterial growth inhibition at concentrations of 250 µg/mL and 500 µg/mL, respectively, as compared with untreated control. Methanol vehicle did not affect H. pylori growth. Conclusion: The observed antibacterial effect of G. glutinosum extracts may be of benefit as an adjuvant treatment of diseases caused by H. pylori.

Tipo de elemento:

Article

Palabras claves no controlados:

Gymnosperma glutinosum, Helicobacter pylori, Methanol extract, Minimal inhibitory concentration (MIC)

Divisiones:

Ciencias Biológicas

Usuario depositante:

Editor Repositorio

Creadores:

Creador	Email	ORCID
Gómez Flores, Ricardo Alberto	ricardo.gomezfl@uanl.edu.mx	NO ESPECIFICADO
Espinosa Ramos, David Alonso	NO ESPECIFICADO	NO ESPECIFICADO
Quintanilla Licea, Ramiro	ramiro.quintanillalc@uanl.edu.mx	orcid.org/0000-0002-4379-6913
Barragán González, María Porfiria	NO ESPECIFICADO	NO ESPECIFICADO
Tamez Guerra, Patricia	NO ESPECIFICADO	orcid.org/0000-0002-4831-2340
Tamez Guerra, Reyes	reyes.tamezgr@uanl.edu.mx	NO ESPECIFICADO
Rodríguez Padilla, Cristina	crrodrig07@gmail.com	orcid.org/0000-0001-5469-8449