Increased Hirsutella citriformis conidia shelf life in Acacia and Hirsutella gum formulations

Flores Villarreal, Rosa Ángela y Orozco Flores, Alonso Alberto y Cantú Bernal, Servando Horacio y Gómez Flores, Ricardo Alberto y Pérez González, Orquídea y Tamez Guerra, Patricia (2023) Increased Hirsutella citriformis conidia shelf life in Acacia and Hirsutella gum formulations. Applied Sciences, 13 (13). p. 7912. ISSN 2076-3417

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URL o página oficial: http://doi.org/10.3390/app13137912

Resumen

: Biological control by beneficial microorganisms is known to significantly reduce the effect of pests on crops yield. Among the biocontrol strategies is the use of entomopathogenic fungi such as Hirsutella citriformis, which has been applied to infect and kill hemipteran insect pests, including Diaphorina citri Kuwayama (Hemiptera: Liviidae) and Bactericera cockerelli Sulc. (Hemiptera: Triozidae). These biological agents are applied in the form of conidia that are often combined with other inert materials to facilitate application, protect conidia, and improve their shelf life. The aim of this study was to implement strategies for developing formulations to increase conidia shelf life. We evaluated gum produced from one strain and conidia from two different H. citriformis strains. Conidia were formulated by evaluating different concentrations of Acacia and Hirsutella gums to enhance conidia viability during storage at 4 ◦C or 25 ◦C. Results indicated that formulations maintained conidia viability for at least 90 d after storage at 25 ◦C (≥70% viability) and at least 120 d after storage at 4 ◦C, which was significantly (p ≤ 0.05) higher than that of the control, without observing changes in pH values. We also demonstrated 100% formulation purity from days 0 to 120, among all treatments. In conclusion, evaluated formulations maintained H. citriformis conidia viability for at least three months, when stored at 4 ◦C.

Tipo de elemento:

Article

Palabras claves no controlados:

Biocontrol, Goma de acacia, Goma de hirsutella, Hongos entomopatógenos, Bioinsectidas, Duración, Formulación de conidias

Materias:

Q Ciencia > QR Microbiología

Divisiones:

Ciencias Biológicas

Usuario depositante:

Editor Repositorio

Creadores:

Creador	Email	ORCID
Flores Villarreal, Rosa Ángela	NO ESPECIFICADO	NO ESPECIFICADO
Orozco Flores, Alonso Alberto	NO ESPECIFICADO	NO ESPECIFICADO
Cantú Bernal, Servando Horacio	NO ESPECIFICADO	NO ESPECIFICADO
Gómez Flores, Ricardo Alberto	ricardo.gomezfl@uanl.edu.mx	NO ESPECIFICADO
Pérez González, Orquídea	NO ESPECIFICADO	NO ESPECIFICADO
Tamez Guerra, Patricia	NO ESPECIFICADO	orcid.org/0000-0002-4831-2340