Comparison of Automated and Manual DNA Isolation Methods of Liquid-Based Cytology Samples

Domínguez Vigil, Irma Guadalupe y Barajas Olmos, Víctor H. y Gallardo Alvarado, Lenny y Pérez Maya, Antonio A. y Garza Rodríguez, María L. y Magallanes Garza, Gerardo I. y Cardona Huerta, Servando y Méndez Lozano, Daniel H. y Vidal Gutiérrez, Oscar y Cantú De León, David F. y Barrera Saldaña, Hugo Alberto (2019) Comparison of Automated and Manual DNA Isolation Methods of Liquid-Based Cytology Samples. Biopreservation and Biobanking, 17 (6). pp. 591-597. ISSN 1947-5535

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Resumen

Liquid-based cytology (LBC) has been used as a diagnostic tool for cervical cancer for years and is now being adopted for other gynecological cancers. LBC represents an important challenge to ensure that the process yields representative biospecimens for quality control (QC) of diagnostic procedures. In this study, we compare QC parameters (integrity, yield and purity, and polymerase chain reaction [PCR] amplification) of DNA isolated from LBC (N= 296) using two different nucleic acid isolation methods, manual (n = 233) or automated (n = 63). We also evaluated two different types of cytological brushes for sampling from the cervix. Our results suggest that manual isolation (yield 22.81 – 1.92 mg) resulted in increased DNA recovery when compared with automated isolation (yield 9.96 – 1.11 mg) from LBC samples, with a p-value of <0.0003. We estimated that 98% (53/54) of the samples preserved the integrity of DNA and were suitable for standard molecular biology analyses. The b-globin gene was amplified in 100% (296/296) of the DNA samples by endpoint PCR. We found no significant difference between the performance of the cytological brushes ( p value of <0.6711) in a general overview. However, when looking at the results from using each brush individually, the manual isolation method was statistically superior to the automated method. Our work illustrates the impact of good QC of preanalytic conditions, which will be important for the application of LBC for developing early detection methods for gynecological cancers.

Tipo de elemento: Article
Palabras claves no controlados: Cáncer de cuello uterino; Citología líquida; Ácidos nucleicos; Control de calidad; Biobanco; Biomarcador.
Materias: R Medicina > RG Ginecología y Obstetricia
Divisiones: Medicina
Usuario depositante: Dr Irma G Dominguez Vigil
Creadores:
CreadorEmailORCID
Domínguez Vigil, Irma GuadalupeNO ESPECIFICADONO ESPECIFICADO
Barajas Olmos, Víctor H.NO ESPECIFICADONO ESPECIFICADO
Gallardo Alvarado, LennyNO ESPECIFICADONO ESPECIFICADO
Pérez Maya, Antonio A.NO ESPECIFICADONO ESPECIFICADO
Garza Rodríguez, María L.NO ESPECIFICADONO ESPECIFICADO
Magallanes Garza, Gerardo I.NO ESPECIFICADONO ESPECIFICADO
Cardona Huerta, ServandoNO ESPECIFICADONO ESPECIFICADO
Méndez Lozano, Daniel H.NO ESPECIFICADONO ESPECIFICADO
Vidal Gutiérrez, OscarNO ESPECIFICADONO ESPECIFICADO
Cantú De León, David F.NO ESPECIFICADONO ESPECIFICADO
Barrera Saldaña, Hugo Albertohabarrera@gmail.comorcid.org/0000-0003-1587-4571
Fecha del depósito: 21 Mar 2024 15:56
Última modificación: 09 Dic 2024 18:07
URI: http://eprints.uanl.mx/id/eprint/27189

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