Genotyping of recombinant Pichia pastoris strains

Viader Salvadó, José María y Cab Barrera, Eddy Luz y Galán Wong, Luis Jesús y Guerrero Olazarán, Martha (2006) Genotyping of recombinant Pichia pastoris strains. Cellular and Molecular Biology Letters, 11 (3). pp. 348-359. ISSN 1689-1392

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URL o página oficial: http://doi.org/10.2478/s11658-006-0029-z

Resumen

A simplified amplified-fragment length polymorphism (AFLP) method was used to genotype Pichia pastoris strains obtained by transformation of P. pastoris strain GS115 with a single integration vector. A total of 14 transformants and 3 control strains were analyzed, which generated 16 different band patterns. A clonal variation was obtained after the transformation process due to genetic differences generated during the transformation event of the host strain. Furthermore, the cluster analysis showed that the transformants with lesser genetic differences with respect to the P. pastoris host strain are the recombinant strains with the highest level of recombinant protein production.

Tipo de elemento:

Article

Palabras claves no controlados:

Pichia pastoris, Polimorfismo de longitud de fragmentos amplificados (AFLP), Variación clonal

Materias:

Q Ciencia > QR Microbiología

Divisiones:

Ciencias Biológicas

Usuario depositante:

Editor Repositorio

Creadores:

Creador	Email	ORCID
Viader Salvadó, José María	NO ESPECIFICADO	NO ESPECIFICADO
Cab Barrera, Eddy Luz	NO ESPECIFICADO	NO ESPECIFICADO
Galán Wong, Luis Jesús	NO ESPECIFICADO	NO ESPECIFICADO
Guerrero Olazarán, Martha	NO ESPECIFICADO	NO ESPECIFICADO