In vitro antitumor activity of endophytic and rhizosphere Gram-positive bacteria from Ibervillea sonorae (S. Watson) Greene against L5178Y-R Lymphoma cells

Romero Arguelles, Ricardo y Romo Sáenz, César Iván y Morán Santibáñez, Karla y Tamez Guerra, Patricia y Quintanilla Licea, Ramiro y Orozco Flores, Alonso Alberto y Ramírez Villalobos, Jesica María y Tamez Guerra, Reyes y Rodríguez Padilla, Cristina y Gomez Flores, Ricardo (2022) In vitro antitumor activity of endophytic and rhizosphere Gram-positive bacteria from Ibervillea sonorae (S. Watson) Greene against L5178Y-R Lymphoma cells. International Journal of Environmental Research and Public Health, 19 (2). pp. 894-905. ISSN 1660-4601

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URL o página oficial: doi:10.3390/ijerph19020894

Resumen

Plant-associated microorganisms represent a potential source of new antitumor compounds. The aim of the present study was to isolate endophytic and rhizosphere Gram-positive bacteria from Ibervillea sonorae and produce extracts with antitumor activity. Methanol and ethyl acetate extracts were obtained from 28 d bacterial fermentation, after which murine L5178Y-R lymphoma cells growth inhibition was evaluated at concentrations ranging from 15.62 µg/mL to 500 µg/mL by the 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide reduction colorimetric assay. IC50 and the selectivity index (SI) were calculated and compared with healthy control human peripheral blood mononuclear cells (PBMC). Identification of the isolated strains was performed using the 16S ribosomal gene and by MALDI-TOF MS mass spectrometry. The endophytic and rhizosphere bacterial extracts from strains ISE-B22, ISE-B26, ISE-B27, ISS-A01, ISS-A06, and ISS-A16 showed significant (p < 0.05) L5178Y-R cell growth inhibition, compared with an untreated control. The rhizosphere Micromonospora echinospora isolate ISS-A16 showed the highest (90.48%) percentage of lymphoma cells growth inhibition and SI (19.1) for PBMC, whereas the Bacillus subtilis ISE-B26 isolate caused significant (p < 0.01) growth inhibition (84.32%) and a SI of 5.2. Taken together, results of the present study evidenced antitumor effects by I. sonorae endophytic and rhizosphere bacteria culture extracts. Further research will involve the elucidation of the compounds that exert the antitumor activity and their evaluation in pre-clinical studies.

Tipo de elemento:

Article

Palabras claves no controlados:

Cucurbitaceae, Ibervillea sonorae, Antitumor, Bacillus, Micromonospora, Endophytic bacteria, Rhizosphere, Gram-positive

Divisiones:

Ciencias Biológicas

Usuario depositante:

Editor Repositorio

Creadores:

Creador	Email	ORCID
Romero Arguelles, Ricardo	ricardoromeroarguelles@gmail.com	orcid.org/0000-0002-6153-7835
Romo Sáenz, César Iván	cesar_ivan_romo@hotmail.com	orcid.org/0000-0002-4013-8974
Morán Santibáñez, Karla	karlita_moran@hotmail.com	orcid.org/0000-0002-3886-8599
Tamez Guerra, Patricia	patamez@hotmail.com	orcid.org/0000-0002-4831-2340
Quintanilla Licea, Ramiro	ramiro.quintanillalc@uanl.edu.mx	orcid.org/0000-0002-4379-6913
Orozco Flores, Alonso Alberto	lacxelo@gmail.com	NO ESPECIFICADO
Ramírez Villalobos, Jesica María	jesicamrv_901@hotmail.com	orcid.org/0000-0002-8595-1459
Tamez Guerra, Reyes	reyes.tamezgr@uanl.edu.mx	NO ESPECIFICADO
Rodríguez Padilla, Cristina	crrodrig07@gmail.com	orcid.org/0000-0001-5469-8449
Gomez Flores, Ricardo	rgomez60@hotmail.com	orcid.org/0000-0002-9518-4433