In Planta Detection of Beauveria bassiana (Ascomycota: Hypocreales) Strains as Endophytes in Bean (Phaseolus vulgaris L.)

Cavazos Vallejo, Teodora y Valadéz Lira, José Alberto y Orozco Flores, Alonso A. y Gómez Flores, Ricardo Alberto y Ek Ramos, María Julissa y Quistián Martínez, Deyanira y Alcocer González, Juan Manuel y Tamez Guerra, Patricia (2023) In Planta Detection of Beauveria bassiana (Ascomycota: Hypocreales) Strains as Endophytes in Bean (Phaseolus vulgaris L.). Plants, 13 (1). pp. 1-17. ISSN 2223-7747

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URL o página oficial: http://doi.org/10.3390/plants13010022

Resumen

Beauveria bassiana (B. bassiana) is a significant entomopathogenic fungus (EPF) in agricultureas a sprayable biocontrol agent. It has the potential to be established as an endophyte (ENP) in various crops, resulting in beneficial effects for the host plants, including resistance to pest insects and increased growth and yield. However, it is not known whether a B. bassiana strain has such a favorable impact on the plant, since it is a common soil microorganism. Therefore, techniques that allow strain monitoring will be advantageous. To date, methods for detecting or monitoring a specific EPF strain after external application are scarce. In the present study, an in planta nested PCR technique was standardized to differentiate between three B. bassiana strains (GHA, PTG4, and BB37) established as endophytes in bean plants under laboratory conditions by detecting the insertion profile of four group I introns located in the 28S gene of B. bassiana ribosomal DNA. This technique recognized a distinct pattern of bands of different sizes for each strain, with a sensitivity of 1 pg per 10 ng of plant DNA. This molecular approach may be more effective monitoring B. bassiana strains after application to evaluate their significance on crops.

Tipo de elemento:

Article

Palabras claves no controlados:

Hongo entomopatógeno; PCR anidada; Frijol; Detección molecular; Intrones del grupo I

Materias:

Q Ciencia > QR Microbiología

Divisiones:

Ciencias Biológicas

Usuario depositante:

Editor Repositorio

Creadores:

Creador	Email	ORCID
Cavazos Vallejo, Teodora	NO ESPECIFICADO	NO ESPECIFICADO
Valadéz Lira, José Alberto	NO ESPECIFICADO	NO ESPECIFICADO
Orozco Flores, Alonso A.	NO ESPECIFICADO	NO ESPECIFICADO
Gómez Flores, Ricardo Alberto	ricardo.gomezfl@uanl.edu.mx	NO ESPECIFICADO
Ek Ramos, María Julissa	maria.ekramos@uanl.edu.mx	NO ESPECIFICADO
Quistián Martínez, Deyanira	NO ESPECIFICADO	NO ESPECIFICADO
Alcocer González, Juan Manuel	NO ESPECIFICADO	NO ESPECIFICADO
Tamez Guerra, Patricia	patamez@hotmail.com	orcid.org/0000-0002-4831-2340