Decellularization of human umbilical arteries

Rodríguez Rodríguez, Víctor Emanuel y Quiroga Garza, Alejandro y Rodríguez Roque, Carlos Saúl y Loera Arias, María de Jesús y Soto Domínguez, Adolfo y Guzmán López, Santos y Vílchez Cavazos, José Félix y Montes de Oca Luna, Roberto y Elizondo Omaña, Rodrigo Enrique (2019) Decellularization of human umbilical arteries. International Journal of Morphology, 37 (1). pp. 111-117. ISSN 0717-9502

Vista previa

Texto
29761.pdf - Versión Publicada
Available under License Creative Commons Attribution Non-commercial No Derivatives.
Download (659kB) | Vista previa

Resumen

Arterial obstruction in small diameter (<6 mm) vessels are many times treated with grafts, however autologous aren’t always available and synthetic have a high rate of complications. Decellularization of umbilical arteries may provide a solution, but the ideal method is debatable. We compare effectiveness between SDS and Triton X-100. Umbilical cords obtained from full term pregnancies with normal development and no evident complications in the newborn, were micro-dissected within 12 h and stored in phosphate buffered saline without freezing. Arteries were then processed for decellularization using 0.1 % and 1 % SDS, and 1 % Triton X100 protocols. Evaluation of cellular and nuclear material, collagen fibers, elastic fibers, and glycosoaminoglycans of the extracellular matrix (ECM) were evaluated as well as morphometric analysis under histological and immunohistochemical techniques. Triton X-100 was ineffective, preserving nuclear remains identified by immunofluorescence, had the most notable damage to elastic fibers, and decrease in collagen. SDS effectively eliminated the nuclei and had a less decrease in elastic fibers and collagen. Laminin was preserved in all groups. No significant differences were identified in luminal diameters; however the middle layer decreased due to decellularization of muscle cells. In conclusion, 0.1 % SDS decellularization was the most effective in eliminating cells and preserving the main components of the ECM.

Tipo de elemento:

Article

Palabras claves no controlados:

Decellularization, Umbilical artery, Vascular graft, Extracellular matrix

Divisiones:

Medicina

Usuario depositante:

Editor Repositorio

Creadores:

Creador	Email	ORCID
Rodríguez Rodríguez, Víctor Emanuel	NO ESPECIFICADO	NO ESPECIFICADO
Quiroga Garza, Alejandro	NO ESPECIFICADO	NO ESPECIFICADO
Rodríguez Roque, Carlos Saúl	NO ESPECIFICADO	NO ESPECIFICADO
Loera Arias, María de Jesús	NO ESPECIFICADO	orcid.org/0000-0001-9561-3624
Soto Domínguez, Adolfo	NO ESPECIFICADO	NO ESPECIFICADO
Guzmán López, Santos	NO ESPECIFICADO	orcid.org/0000-0003-4100-1360
Vílchez Cavazos, José Félix	NO ESPECIFICADO	NO ESPECIFICADO
Montes de Oca Luna, Roberto	NO ESPECIFICADO	NO ESPECIFICADO
Elizondo Omaña, Rodrigo Enrique	rod_omana@yahoo.com	NO ESPECIFICADO