Design and Characterization of {pMyc}/{pMax} Peptide-Coupled Gold Nanosystems for Targeting Myc in Prostate Cancer Cell Lines

Longoria García, Samuel y Sánchez Domínguez, Celia N. y Sánchez Domínguez, Margarita y Delgado Balderas, Jesús R. y Islas Cisneros, José F. y Vidal Gutiérrez, Oscar y Gallardo Blanco, Hugo L. (2023) Design and Characterization of {pMyc}/{pMax} Peptide-Coupled Gold Nanosystems for Targeting Myc in Prostate Cancer Cell Lines. Nanomaterials, 13 (20). pp. 1-6. ISSN 2079-4991

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URL o página oficial: https://www.mdpi.com/2079-4991/13/20/2802

Resumen

Myc and Max are essential proteins in the development of prostate cancer. They act by dimerizing and binding to E-box sequences. Disrupting the Myc:Max heterodimer interaction or its binding to E-box sequences to interrupt gene transcription represent promising strategies for treating cancer. We designed novel {pMyc} and {pMax} peptides from reference sequences, and we evaluated their ability to bind specifically to E-box sequences using an electrophoretic mobility shift assay ({EMSA}). Then, we assembled nanosystems ({NSs}) by coupling {pMyc} and {pMax} peptides to {AuNPs}, and determined peptide conjugation using {UV}-Vis spectroscopy. After that, we characterized the {NS} to obtain the nanoparticle's size, hydrodynamic diameter, and zeta potential. Finally, we evaluated hemocompatibility and cytotoxic effects in three different prostate adenocarcinoma cell lines ({LNCaP}, {PC}-3, and {DU}145) and a non-cancerous cell line (Vero {CCL}-81). {EMSA} results suggests peptide-nucleic acid interactions between the {pMyc}:{pMax} dimer and the E-box. The hemolysis test showed little hemolytic activity for the {NS} at the concentrations (5, 0.5, and 0.05 ng/uL) we evaluated. Cell viability assays showed {NS} cytotoxicity. Overall, results suggest that the {NS} with {pMyc} and {pMax} peptides might be suitable for further research regarding Myc-driven prostate adenocarcinomas.

Tipo de elemento:

Article

Palabras claves no controlados:

AuNPs; Max; Myc; Cáncer; Cúmulos de nanopartículas coloidales; Nanocomplejos; Nanomedicina; Péptidos

Materias:

R Medicina > RC Medicina Interna, Psiquiatría, Neurología

Divisiones:

Medicina

Usuario depositante:

Dr.C. Hugo Gallardo Blanco

Creadores:

Creador	Email	ORCID
Longoria García, Samuel	samuel.longoriagr@uanl.edu.mx	orcid.org/0000-0002-4056-5343
Sánchez Domínguez, Celia N.	celia.sanchezdm@uanl.edu.mx	orcid.org/0000-0002-3444-9565
Sánchez Domínguez, Margarita	margarita.sanchez@cimav.edu.mx	orcid.org/0000-0003-4755-4441
Delgado Balderas, Jesús R.	jrolandodelgadob@gmail.com	orcid.org/0000-0002-9016-2016
Islas Cisneros, José F.	NO ESPECIFICADO	NO ESPECIFICADO
Vidal Gutiérrez, Oscar	oscar.vidalgtz@uanl.edu.mx	orcid.org/0000-0003-3723-8525
Gallardo Blanco, Hugo L.	hugo.gallardobl@uanl.edu.mx	orcid.org/0000-0002-7816-4967