Impact of Methodological Variations on {MTT} Assay for Gold Nanoparticle Toxicity Evaluation

Martínez Saraoz, Jessica Victoria y Longoria García, Samuel y Gallardo Blanco, Hugo Leonid y Sánchez Domínguez, Celia Nohemí y Sánchez Domínguez, Margarita (2025) Impact of Methodological Variations on {MTT} Assay for Gold Nanoparticle Toxicity Evaluation. In: A Cross-Disciplinary Exploration of {STEM}. Springer Nature Switzerland, pp. 1-10. ISBN 978-3-031-99986-4 978-3-031-99987-1

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URL o página oficial: https://link.springer.com/10.1007/978-3-031-99987-...

Resumen

Gold nanoparticles (AuNPs) coated with PEI-based copolymers have emerged as a promising non-viral vector for gene therapy due to their exceptional biocompatibility and efficient gene delivery capabilities. Green synthesis methods, which utilize environmentally friendly and sustainable approaches, have gained significant attention for producing biocompatible nanoparticles. While AuNPs have been extensively studied, challenges often arise when conducting cell viability assays, particularly using the MTT (3-(4,5-dimethylthiazol-2-yl)- 2,5-diphenyltetrazolium bromide) assay. The inherent properties of nanoparticles,such as their size, shape, and surface chemistry, can interfere with the MTT assay’s mechanism, leading to inaccurate assessments of cell viability. Additionally, the interaction between the MTT reagent and the nanoparticles can further complicate the assay and potentially yield misleading results. To address these challenges, this study aims to optimize AuNP synthesis, establish optimal MTT assay conditions and mitigate interference effects by carefully controlling the synthesis parameters, optimizing the MTT assay conditions, and addressing the potential interference effects. This study aims to provide a reliable and accurate method for evaluating the cytotoxicity and biocompatibility of AuNP-based gene delivery systems. This knowledge will contribute to the development of safe and effective nanomedicine for various biomedical applications.

Tipo de elemento:

Sección de libro.

Palabras claves no controlados:

Metal Nanoparticles, Gold, Cell Survival, Toxicity Tests, Reproducibility of Results, Cell Line

Materias:

CONACYT > Biología y Química
CONACYT > Ingeniería y Tecnología
CONACYT > Medicina y Ciencias de la Salud

Divisiones:

Medicina

Usuario depositante:

Dr.C. Hugo Gallardo Blanco

Creadores:

Creador	Email	ORCID
Martínez Saraoz, Jessica Victoria	NO ESPECIFICADO	NO ESPECIFICADO
Longoria García, Samuel	samuel.longoriagr@uanl.edu.mx	orcid.org/0000-0002-4056-5343
Gallardo Blanco, Hugo Leonid	hugo.gallardobl@uanl.edu.mx	orcid.org/0000-0002-7816-4967
Sánchez Domínguez, Celia Nohemí	celia.sanchezdm@uanl.edu.mx	orcid.org/0000-0002-3444-9565
Sánchez Domínguez, Margarita	margarita.sanchez@cimav.edu.mx	orcid.org/0000-0003-4755-4441