High-Level Expression of Recombinant Bovine Lactoferrin in Pichia pastoris with Antimicrobial Activity

Iglesias Figueroa, Blanca y Valdiviezo Godina, Norberto y Siqueiros Cendón, Tania y Sinagawa García, Sugey Ramona y Arévalo Gallegos, Sigifredo y Rascón Cruz, Quintín (2016) High-Level Expression of Recombinant Bovine Lactoferrin in Pichia pastoris with Antimicrobial Activity. International Journal of Molecular Sciences, 17 (6). pp. 1-11. ISSN 1422-0067

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URL o página oficial: http://doi.org/10.3390/ijms17060902

Resumen

In this study, bovine lactoferrin (bLf), an iron-binding glycoprotein considered an important nutraceutical protein because of its several properties, was expressed in Pichia pastoris KM71-H under AOX1 promoter control, using pJ902 as the recombinant plasmid. Dot blotting analysis revealed the expression of recombinant bovine lactoferrin (rbLf) in Pichia pastoris. After Bach fermentation and purification by molecular exclusion, we obtained an expression yield of 3.5 g/L of rbLf. rbLf and predominantly pepsin-digested rbLf (rbLfcin) demonstrated antibacterial activity against Escherichia coli (E. coli) BL21DE3, Staphylococcus aureus (S. aureus) FRI137, and, in a smaller percentage, Pseudomonas aeruginosa (Ps. Aeruginosa) ATCC 27833. The successful expression and characterization of functional rbLf expressed in Pichia pastoris opens a prospect for the development of natural antimicrobial agents produced recombinantly.

Tipo de elemento: Article
Palabras claves no controlados: Recombinant bovine lactoferrin, Lactoferricin, Antibacterial activity, Pichia pastoris
Materias: Q Ciencia > QD Química
Divisiones: Ciencias Químicas
Usuario depositante: Editor Repositorio
Creadores:
CreadorEmailORCID
Iglesias Figueroa, BlancaNO ESPECIFICADONO ESPECIFICADO
Valdiviezo Godina, NorbertoNO ESPECIFICADONO ESPECIFICADO
Siqueiros Cendón, TaniaNO ESPECIFICADONO ESPECIFICADO
Sinagawa García, Sugey RamonaNO ESPECIFICADONO ESPECIFICADO
Arévalo Gallegos, SigifredoNO ESPECIFICADONO ESPECIFICADO
Rascón Cruz, QuintínNO ESPECIFICADONO ESPECIFICADO
Fecha del depósito: 13 Mayo 2020 15:30
Última modificación: 10 Jun 2020 20:08
URI: http://eprints.uanl.mx/id/eprint/14789

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