Antitumor activity of Chlorella sorokiniana and Scenedesmus sp. microalgae native of Nuevo León State, México

Reyna Martinez, Raul y Gómez Flores, Ricardo Alberto y Lopez Chuken, Ulrico Javier y Quintanilla Licea, Ramiro y Caballero Hernández, Diana Elia y Rodríguez Padilla, María Cristina y Beltrán Rocha, Julio César y Tamez Guerra, Patricia (2018) Antitumor activity of Chlorella sorokiniana and Scenedesmus sp. microalgae native of Nuevo León State, México. PeerJ, 6. pp. 1-15. ISSN 2167-8359

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URL o página oficial: http://doi.org/10.7717/peerj.4358

Resumen

ABSTRACT Cancer cases result in 13% of all deaths worldwide. Unwanted side effects in patients underconventionaltreatmentshaveledtothesearchforbeneficialalternativetherapies. Microalgae synthesize compounds with known invitro and invivo biological activity againstdifferenttumorcelllines.Therefore,nativemicroalgaefromtheStateofNuevo Leon, Mexico may become a potential source of antitumor agents. The aim of the present study was to evaluate the invitro cytotoxic effect of Nuevo Leon regional Chlorella sorokiniana (Chlorellales: Chlorellaceae) and Scenedesmus sp. (Chlorococcales: Scenedesmaceae). Native microalgae crude organic extracts cytotoxicity against murine L5178Y-R lymphoma cell line and normal lymphocyte proliferation were evaluatedusingtheMTTreductioncolorimetricassay.Celldeathpathwaywasanalyzed byacridineorangeandethidiumbromidestaining,DNAdegradationin2%agarosegel electrophoresis and caspases activity. Results indicated significant (p<0.05) 61.89% ±3.26% and 74.77%±1.84% tumor cytotoxicity by C.sorokiniana and Scenedesmus sp.methanolextracts,respectively,at500 µg/mL,bythemechanismofapoptosis.This studycontributestoMexicanmicroalgaebiodiversityknowledgeandtheirpotentialas antitumor agent sources.

Tipo de elemento:

Article

Divisiones:

Ciencias Químicas

Usuario depositante:

Lic. Josimar Pulido

Creadores:

Creador	Email	ORCID
Reyna Martinez, Raul	NO ESPECIFICADO	NO ESPECIFICADO
Gómez Flores, Ricardo Alberto	ricardo.gomezfl@uanl.edu.mx	NO ESPECIFICADO
Lopez Chuken, Ulrico Javier	NO ESPECIFICADO	NO ESPECIFICADO
Quintanilla Licea, Ramiro	NO ESPECIFICADO	NO ESPECIFICADO
Caballero Hernández, Diana Elia	NO ESPECIFICADO	NO ESPECIFICADO
Rodríguez Padilla, María Cristina	cristina.rodriguezpd@uanl.edu.mx	orcid.org/0000-0001-5469-8449
Beltrán Rocha, Julio César	NO ESPECIFICADO	NO ESPECIFICADO
Tamez Guerra, Patricia	NO ESPECIFICADO	orcid.org/0000-0002-4831-2340