Ectopic BAT mUCP-1 overexpression in SKM by delivering a BMP7/PRDM16/PGC-1a gene cocktail or single PRMD16 using non-viral UTMD gene therapy.

Chen, Shuyuan y Bastarrachea, Raúl A. y Shen, Jin Song y Laviada Nagel, Antonio y Rodríguez Ayala, Ernesto y Nava González, Edna Judith y Huang, Pintong y DeFronzo, Ralph A. y Kent, Jack W. y Grayburn, Paul A. (2018) Ectopic BAT mUCP-1 overexpression in SKM by delivering a BMP7/PRDM16/PGC-1a gene cocktail or single PRMD16 using non-viral UTMD gene therapy. Gene Therapy, 25 (7). pp. 497-509. ISSN 0969-7128

Texto (Progress in inducing an ectopic brown adipose tissue (BAT) phenotype in skeletal muscle (SKM).)
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Resumen

Here we present our progress in inducing an ectopic brown adipose tissue (BAT) phenotype in skeletal muscle (SKM) as a potential gene therapy for obesity and its comorbidities. We used ultrasound-targeted microbubble destruction (UTMD), a novel targeted, non-viral approach to gene therapy, to deliver genes in the BAT differentiation pathway into rodent SKM to engineer a thermogenic BAT phenotype with ectopic mUCP-1 overexpression. In parallel, we performed a second protocol using wild-type Ucp-1-null knockout mice to test whether the effects of the gene therapy are UCP-1 dependent. Our main findings were a robust cellular presence of mUCP-1 immunostaining (IHC), significantly higher expression levels of mUCP-1 measured by qRT-PCR, and highest temperature elevation measured by infrared thermography in the treated thigh, achieved in rats after delivering the UTMD-PRDM16/PGC-1a/BMP7/hyPB gene cocktail. Interestingly, the weight loss obtained in the treated rats with the triple gene delivery, never recovered the levels observed in the controls in spite of food intake recovery. Our results establish the feasibility of minimally invasive UTMD gene-based therapy administration in SKM, to induce overexpression of ectopic mUCP-1 after delivery of the thermogenic BAT gene program, and describe systemic effects of this intervention on food intake, weight loss, and thermogenesis.

Tipo de elemento:

Article

Materias:

R Medicina > R Medicina en General

Divisiones:

Salud Pública y Nutrición

Usuario depositante:

Dra. Edna J. Nava-González

Creadores:

Creador	Email	ORCID
Chen, Shuyuan	NO ESPECIFICADO	NO ESPECIFICADO
Bastarrachea, Raúl A.	NO ESPECIFICADO	NO ESPECIFICADO
Shen, Jin Song	NO ESPECIFICADO	NO ESPECIFICADO
Laviada Nagel, Antonio	NO ESPECIFICADO	NO ESPECIFICADO
Rodríguez Ayala, Ernesto	NO ESPECIFICADO	NO ESPECIFICADO
Nava González, Edna Judith	edna.navag@uanl.mx	orcid.org/0000-0001-8818-2600
Huang, Pintong	NO ESPECIFICADO	NO ESPECIFICADO
DeFronzo, Ralph A.	NO ESPECIFICADO	NO ESPECIFICADO
Kent, Jack W.	NO ESPECIFICADO	NO ESPECIFICADO
Grayburn, Paul A.	NO ESPECIFICADO	NO ESPECIFICADO