Measuring of Mycobacterium tuberculosis growth: a correlation of the optical measurements with colony forming units

Peñuelas Urquides, Katia y Villarreal Treviño, Licet y Silva Ramírez, Beatriz y Rivadeneyra Espinoza, Liliana y Said Fernández, Salvador y León, Mario Bermúdez de (2013) Measuring of Mycobacterium tuberculosis growth: a correlation of the optical measurements with colony forming units. Brazilian journal of microbiology, 44 (1). pp. 287-290. ISSN 1517-8382

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URL o página oficial: http://doi.org/10.1590/S1517-83822013000100042

Resumen

The quantification of colony forming units (cfu), turbidity, and optical density at 600 nm (OD600) measurements were used to evaluate Mycobacterium tuberculosis growth. Turbidity and OD600 measurements displayed similar growth curves, while cfu quantification showed a continuous growth curve. We determined the cfu equivalents to McFarland and OD600 units.

Tipo de elemento:

Article

Palabras claves no controlados:

bacterial growth, colony forming units, McFarland, Mycobacterium tuberculosis, optical density

Divisiones:

Ciencias Biológicas
Medicina

Usuario depositante:

Lic. Josimar Pulido

Creadores:

Creador	Email	ORCID
Peñuelas Urquides, Katia	NO ESPECIFICADO	NO ESPECIFICADO
Villarreal Treviño, Licet	NO ESPECIFICADO	NO ESPECIFICADO
Silva Ramírez, Beatriz	NO ESPECIFICADO	NO ESPECIFICADO
Rivadeneyra Espinoza, Liliana	NO ESPECIFICADO	NO ESPECIFICADO
Said Fernández, Salvador	NO ESPECIFICADO	NO ESPECIFICADO
León, Mario Bermúdez de	NO ESPECIFICADO	NO ESPECIFICADO