Organotypic culture of breast tumor explants as a multicellular system for the screening of natural compounds with antineoplastic potential

Carranza Torres, Irma Edith y Guzmán Delgado, Nancy Elena y Coronado Martínez, Consuelo y Bañuelos García, José Inocente y Viveros Valdez, José Ezequiel y Morán Martínez, Javier y Carranza Rosales, María del Pilar (2015) Organotypic culture of breast tumor explants as a multicellular system for the screening of natural compounds with antineoplastic potential. BioMed Research International, 2015. pp. 1-13. ISSN 2314-6133

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URL o página oficial: http://doi.org/10.1155/2015/618021

Resumen

Breast cancer is the leading cause of death in women worldwide. The search for novel compounds with antitumor activity, with less adverse effects and higher efficacy, and the development of methods to evaluate their toxicity is an area of intense research. In this study we implemented the preparation and culture of breast tumor explants, which were obtained from precision-cut breast tumor slices. In order to validate the model we are proposing to screen antineoplastic effect of natural compounds, we selected caffeic acid, ursolic acid, and rosmarinic acid. Using the Krumdieck tissue slicer, precision-cut tissue slices were prepared from breast cancer samples; from these slices, 4 mm explants were obtained and incubated with the selected compounds. Viability was assessed by Alamar Blue assay, LDH release, and histopathological criteria. Results showed that the viability of the explants cultured in the presence of paclitaxel (positive control) decreased significantly (

Tipo de elemento: Article
Materias: Q Ciencia > QR Microbiología
R Medicina > RC Medicina Interna, Psiquiatría, Neurología
Divisiones: Ciencias Biológicas
Usuario depositante: Lic. Jesús E. Alvarado
Creadores:
CreadorEmailORCID
Carranza Torres, Irma EdithNO ESPECIFICADONO ESPECIFICADO
Guzmán Delgado, Nancy ElenaNO ESPECIFICADONO ESPECIFICADO
Coronado Martínez, ConsueloNO ESPECIFICADONO ESPECIFICADO
Bañuelos García, José InocenteNO ESPECIFICADONO ESPECIFICADO
Viveros Valdez, José Ezequieljose.viverosvld@uanl.edu.mxorcid.org/0000-0001-5944-7972
Morán Martínez, JavierNO ESPECIFICADONO ESPECIFICADO
Carranza Rosales, María del PilarNO ESPECIFICADONO ESPECIFICADO
Fecha del depósito: 12 Abr 2019 19:13
Última modificación: 04 Jul 2019 15:09
URI: http://eprints.uanl.mx/id/eprint/15113

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