Organotypic culture of breast tumor explants as a multicellular system for the screening of natural compounds with antineoplastic potential

Carranza Torres, Irma Edith y Guzmán Delgado, Nancy Elena y Coronado Martínez, Consuelo y Bañuelos García, José Inocente y Viveros Valdez, José Ezequiel y Morán Martínez, Javier y Carranza Rosales, María del Pilar (2015) Organotypic culture of breast tumor explants as a multicellular system for the screening of natural compounds with antineoplastic potential. BioMed Research International, 2015. pp. 1-13. ISSN 2314-6133

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URL o página oficial: http://doi.org/10.1155/2015/618021

Resumen

Breast cancer is the leading cause of death in women worldwide. The search for novel compounds with antitumor activity, with less adverse effects and higher efficacy, and the development of methods to evaluate their toxicity is an area of intense research. In this study we implemented the preparation and culture of breast tumor explants, which were obtained from precision-cut breast tumor slices. In order to validate the model we are proposing to screen antineoplastic effect of natural compounds, we selected caffeic acid, ursolic acid, and rosmarinic acid. Using the Krumdieck tissue slicer, precision-cut tissue slices were prepared from breast cancer samples; from these slices, 4 mm explants were obtained and incubated with the selected compounds. Viability was assessed by Alamar Blue assay, LDH release, and histopathological criteria. Results showed that the viability of the explants cultured in the presence of paclitaxel (positive control) decreased significantly (

Tipo de elemento:

Article

Materias:

Q Ciencia > QR Microbiología
R Medicina > RC Medicina Interna, Psiquiatría, Neurología

Divisiones:

Ciencias Biológicas

Usuario depositante:

Editor Repositorio

Creadores:

Creador	Email	ORCID
Carranza Torres, Irma Edith	NO ESPECIFICADO	NO ESPECIFICADO
Guzmán Delgado, Nancy Elena	NO ESPECIFICADO	NO ESPECIFICADO
Coronado Martínez, Consuelo	NO ESPECIFICADO	NO ESPECIFICADO
Bañuelos García, José Inocente	NO ESPECIFICADO	NO ESPECIFICADO
Viveros Valdez, José Ezequiel	jose.viverosvld@uanl.edu.mx	orcid.org/0000-0001-5944-7972
Morán Martínez, Javier	NO ESPECIFICADO	NO ESPECIFICADO
Carranza Rosales, María del Pilar	NO ESPECIFICADO	NO ESPECIFICADO