Molecular Identification, Antifungal Susceptibility, and Geographic Origin of Clinical Strains of Sporothrix schenckii Complex in Mexico

Rojas, Olga y Bonifaz, Alexandro y Campos, Christian y Treviño Rangel, Rogelio y González Álvarez, Rafael y González, Gloria (2018) Molecular Identification, Antifungal Susceptibility, and Geographic Origin of Clinical Strains of Sporothrix schenckii Complex in Mexico. Journal of Fungi, 4 (3). pp. 1-9. ISSN 2309-608X

[img]
Vista previa
Texto
80.pdf - Versión Publicada
Available under License Creative Commons Attribution Non-commercial No Derivatives.

Download (1MB) | Vista previa
URL o página oficial: http://doi.org/10.3390/jof4030086

Resumen

Abstract:SporotrichosisisasubcutaneousmycosiscausedbySporothrixschenckiicomplex. Thedisease hasbeenreportedworldwide.However,theincidenceoftheetiologicalagentvariesinitsgeographic distribution. We studied 39 clinical isolates of Sporothrix schenckii from diverse regions in Mexico, collectedfrom1998to2016.Molecularidentificationwasperformedbysequenceanalysisofthepartial calmodulin gene. In vitro antifungal susceptibility to amphotericin B (AMB), itraconazole (ITC), voriconazole (VRC), posaconazole (PSC), fluconazole (FLC), terbinafine (TRB), caspofungin (CSF), anidulafungin (ANF), and micafungin (MCF) was evaluated. Thirty-eight isolates of S. schenckii complexweredividedintofivesupportedcladesinaphylogenetictree. Thepredominantclinicalform waslymphocutaneous(92.3%),fixedcutaneous(5.1%),anddisseminated(2.5%). Terbinafineexhibited the best in vitro antifungal activity, while fluconazole was ineffective against Sporothrix schenckii complex. Our results showed diverse geographic distribution of clinical isolates in eight states; definitive identification was done by CAL gen PCR-sequencing. In Mexico, S. schenckii is considered to be an etiological agent of human sporotrichosis cases, and lymphocutaneous is the most prevalent form of the disease. This study revealed four clades of S. schenckii sensu stricto by phylogenetic analysis. Furthermore, we report one case of S. globosa isolated from human origin from the North of Mexico.

Tipo de elemento: Article
Materias: Q Ciencia > QR Microbiología
Divisiones: Medicina
Usuario depositante: Lic. Josimar Pulido
Creadores:
CreadorEmailORCID
Rojas, OlgaNO ESPECIFICADONO ESPECIFICADO
Bonifaz, AlexandroNO ESPECIFICADONO ESPECIFICADO
Campos, ChristianNO ESPECIFICADONO ESPECIFICADO
Treviño Rangel, RogelioNO ESPECIFICADONO ESPECIFICADO
González Álvarez, RafaelNO ESPECIFICADONO ESPECIFICADO
González, GloriaNO ESPECIFICADONO ESPECIFICADO
Fecha del depósito: 17 Jun 2020 16:35
Última modificación: 17 Jun 2020 16:35
URI: http://eprints.uanl.mx/id/eprint/16106

Actions (login required)

Ver elemento Ver elemento

Downloads

Downloads per month over past year