Molecular Identification, Antifungal Susceptibility, and Geographic Origin of Clinical Strains of Sporothrix schenckii Complex in Mexico

Rojas, Olga y Bonifaz, Alexandro y Campos, Christian y Treviño Rangel, Rogelio de Jesús y González Álvarez, Rafael y González González, Gloria María (2018) Molecular Identification, Antifungal Susceptibility, and Geographic Origin of Clinical Strains of Sporothrix schenckii Complex in Mexico. Journal of Fungi, 4 (3). pp. 1-9. ISSN 2309-608X

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Resumen

Abstract:SporotrichosisisasubcutaneousmycosiscausedbySporothrixschenckiicomplex. Thedisease asbeenreportedworldwide.However,theincidenceoftheetiologicalagentvariesinitsgeographic distribution. We studied 39 clinical isolates of Sporothrix schenckii from diverse regions in Mexico, collectedfrom1998to2016.Molecularidentificationwasperformedbysequenceanalysisofthepartial calmodulin gene. In vitro antifungal susceptibility to amphotericin B (AMB), itraconazole (ITC), voriconazole (VRC), posaconazole (PSC), fluconazole (FLC), terbinafine (TRB), caspofungin (CSF), anidulafungin (ANF), and micafungin (MCF) was evaluated. Thirty-eight isolates of S. schenckii complexweredividedintofivesupportedcladesinaphylogenetictree. Thepredominantclinicalform waslymphocutaneous(92.3%),fixedcutaneous(5.1%),anddisseminated(2.5%). Terbinafineexhibited the best in vitro antifungal activity, while fluconazole was ineffective against Sporothrix schenckii complex. Our results showed diverse geographic distribution of clinical isolates in eight states; definitive identification was done by CAL gen PCR-sequencing. In Mexico, S. schenckii is considered to be an etiological agent of human sporotrichosis cases, and lymphocutaneous is the most prevalent form of the disease. This study revealed four clades of S. schenckii sensu stricto by phylogenetic analysis. Furthermore, we report one case of S. globosa isolated from human origin from the North of Mexico.

Tipo de elemento:	Article
Materias:	Q Ciencia > QR Microbiología
Divisiones:	Medicina
Usuario depositante:	Lic. Josimar Pulido
Creadores:	Creador Email ORCID Rojas, Olga NO ESPECIFICADO NO ESPECIFICADO Bonifaz, Alexandro NO ESPECIFICADO NO ESPECIFICADO Campos, Christian NO ESPECIFICADO NO ESPECIFICADO Treviño Rangel, Rogelio de Jesús NO ESPECIFICADO orcid.org/0000-0002-4433-6556 González Álvarez, Rafael NO ESPECIFICADO NO ESPECIFICADO González González, Gloria María NO ESPECIFICADO NO ESPECIFICADO
Fecha del depósito:	17 Jun 2020 16:35
Última modificación:	05 Mar 2024 17:51
URI:	http://eprints.uanl.mx/id/eprint/16106

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