Development of a Novel Scaffold of Chitosan, Type IV Collagen and Integrin α3β1 As Alternative Scaffold for Primary Culture of Podocytes

Zárate Triviño, Diana y Hernández Martínez, Sara y Bollain y Goytia de la Rosa, Juan y Franco Molina, Moisés y Elizalde Peña, Eduardo y Hernández Villegas, Margarita y Rangel Ochoa, Gloria y Rodríguez Padilla, Cristina (2018) Development of a Novel Scaffold of Chitosan, Type IV Collagen and Integrin α3β1 As Alternative Scaffold for Primary Culture of Podocytes. Applied Sciences, 8 (6). pp. 1-13. ISSN 2076-3417

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URL o página oficial: http://doi.org/10.3390/app8060930

Resumen

Loss of podocytes has been a main pathology present in renal diseases; the leak of these specialized cells increases the permeability of the glomerular basal membrane (GMB) and protein release affecting the glomeruli, the main structure of the kidney. The study of different physiopathology mechanism has been a challenge because of the short lifetime of podocytes in vitro. We obtained and characterized composites based on Chitosan (CTS), polyvinyl alcohol (PVA), type IV collagen and integrin α3β1 as a possible application in primary culture of podocytes. Podocytes were extracted from the urine of patients with Idiopathic Nephrotic Syndrome (INS). To evaluate biocompatibility, we assessed cell viability through the lactate dehydrogenase assay. Immunohistochemical staining was used to detect the expression of specific proteins from podocytes such as podocin, and podocalyxin and CD80, a marker of cellular stress. The results showed that our synthesis method promotes the copolymerization of the components in the scaffold. Due to its reactivity, the amine group of chitosan made links with type IV collagen and integrin α3β1. The swelling and degradation tests allowed us to select the material with the best mechanical properties for cellular culture. The expression of podocin and podocalyxin remains the same in the culture of podocytes on the scaffold; in contrast, CD80 expression increased. The viability of podocytes cultured on the CTS/PVA/type IV collagen/integrin α3β1 scaffold increased in comparison to the culture control. Keywords: podocytes; half-life; chitosan; protein expression; scaffold

Tipo de elemento: Article
Materias: Q Ciencia > Q Ciencias en General
T Tecnología > T Tecnología en General
Divisiones: Ciencias Biológicas
Usuario depositante: Lic. Josimar Pulido
Creadores:
CreadorEmailORCID
Zárate Triviño, DianaNO ESPECIFICADONO ESPECIFICADO
Hernández Martínez, SaraNO ESPECIFICADONO ESPECIFICADO
Bollain y Goytia de la Rosa, JuanNO ESPECIFICADONO ESPECIFICADO
Franco Molina, MoisésNO ESPECIFICADONO ESPECIFICADO
Elizalde Peña, EduardoNO ESPECIFICADONO ESPECIFICADO
Hernández Villegas, MargaritaNO ESPECIFICADONO ESPECIFICADO
Rangel Ochoa, GloriaNO ESPECIFICADONO ESPECIFICADO
Rodríguez Padilla, CristinaNO ESPECIFICADONO ESPECIFICADO
Fecha del depósito: 24 Jun 2020 13:13
Última modificación: 24 Jun 2020 13:13
URI: http://eprints.uanl.mx/id/eprint/16205

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