Detection of aedes aegypti mosquitoes infected with dengue virus as a complementary method for increasing the sensitivity of surveillance: identification of serotypes 1, 2, and 4 by rt-pcr in Quintana Roo, Mexico

Mendez Galvan, Jorge y Sánchez Casas, Rosa María y Gaitan Burns, Alejandro y Diaz Gonzalez, Esteban E. y Ibarra Juárez, Luis Arturo y Medina de la Garza, Carlos Eduardo y Domínguez Galera, Marco Antonio y Mis Ávila, Pedro y Fernández Salas, Ildefonso (2014) Detection of aedes aegypti mosquitoes infected with dengue virus as a complementary method for increasing the sensitivity of surveillance: identification of serotypes 1, 2, and 4 by rt-pcr in Quintana Roo, Mexico. The Southwestern entomologist, 39 (2). pp. 307-316. ISSN 0147-1724

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Abstract. Sensitivity of monitoring Aedes aegypti (L.) populations was determined to identify the distribution of dengue virus (DENV) during epidemics in Quintana Roo. From September to November 2012, we used a motorized aspirator to collect 2,144 female Ae. aegypti from 569 homes. These were grouped into 220 to use semi-nested RT-PCR for DENV, and positive groups were analyzed individually. Five groups (2.27%) were positive for DENV. Individual analysis yielded eight groups that tested positive, six with DENV-2, one DENV-1, and one DENV-4. The latter was not reported by the surveillance system that year. The mean number of female mosquitoes per household was 3.77 ± 5.71, and the rate of viral infection of Ae. aegypti was 0.4%. Most infected mosquitoes (49%) were concentrated in 10% of the houses. Monitoring Ae. aegypti infected with DENV has the potential to complement the current system of clinical and entomological surveillance.

Tipo de elemento: Article
Materias: Q Ciencia > Q Ciencias en General
Q Ciencia > QH Historia Natural, Biología
Divisiones: Ciencias Biológicas
Usuario depositante: Dra Rosa Maria / RM Sanchez Casas
Creadores:
CreadorEmailORCID
Mendez Galvan, JorgeNO ESPECIFICADONO ESPECIFICADO
Sánchez Casas, Rosa MaríaNO ESPECIFICADONO ESPECIFICADO
Gaitan Burns, AlejandroNO ESPECIFICADONO ESPECIFICADO
Diaz Gonzalez, Esteban E.NO ESPECIFICADONO ESPECIFICADO
Ibarra Juárez, Luis ArturoNO ESPECIFICADONO ESPECIFICADO
Medina de la Garza, Carlos EduardoNO ESPECIFICADONO ESPECIFICADO
Domínguez Galera, Marco AntonioNO ESPECIFICADONO ESPECIFICADO
Mis Ávila, PedroNO ESPECIFICADONO ESPECIFICADO
Fernández Salas, Ildefonsoifernand1@hotmail.comNO ESPECIFICADO
Fecha del depósito: 31 Jul 2015 21:00
Última modificación: 05 Dic 2016 16:45
URI: http://eprints.uanl.mx/id/eprint/6437

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