Detection of dengue virus serotype 2 in aedes aegypti in Quintana Roo, Mexico, 2011

Sánchez Casas, Rosa María y Alpuche Delgado, Rafael H. y Blitvich, Bradley J. y Gonzalez Diaz, Esteban E. y Ramírez Jiménez, Rocío y Zárate Nahón, Erwy Arvid y Sánchez Rodríguez, Olga Saraí y Laguna Aguilar, Maricela y Alvarado Moreno, Marcela Selene y Ibarra Juárez, Luis Arturo y Medina de la Garza, Carlos Eduardo y Loroño Pino, María Alba y Domínguez Galera, Marco Antonio y Mis Ávila, Pedro y Fernández Salas, Ildefonso (2013) Detection of dengue virus serotype 2 in aedes aegypti in Quintana Roo, Mexico, 2011. Southwestern entomologist, 38 (1). pp. 109-117. ISSN 0147-1724

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Resumen

Abstract. In October 2011, the State Health Department announced that several laboratory-confirmed cases of dengue had occurred among residents in two neighborhoods of Benito Juarez, Quintana Roo State, Mexico. To identify the dengue virus serotype(s) temporally and spatially associated with the cases, entomologic-based virus surveillance was initiated in October 2011 in both neighborhoods. Adult mosquitoes were collected from 88 houses by CDCbackpack aspirator, and all female Aedes aegypti L. (n = 419) were individually homogenized and assayed in pools of as many as 10 by reverse transcriptionpolymerase chain reaction (RT-PCR) using dengue virus-specific primers. Five (12%) of 41 pools were positive for dengue virus RNA. The individual mosquitoes that comprised the pools were analyzed separately by RT-PCR using dengue virus serotype-specific primers. Six mosquitoes were positive for dengue virus serotype-2 (DENV-2) RNA, three of which were collected in the same house. The mean number of female Ae. aegypti collected in each house was 4.76 ± 6.19. The overall dengue virus-infection rate in female Ae. aegypti was 1.4%. Interestingly, most (60%) of mosquito females were collected only from 15 (17%) houses. In summary, we provide evidence of recent DENV-2 transmission in Quintana Roo State.

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